Subchloroplastic localization of NAD kinase activity: evidence for a Ca2+, calmodulin-dependent activity at the envelope and for a Ca2+, calmodulin-independent activity in the stroma of pea chloroplasts

Article Article scientifique Article Subchloroplastic localization of NAD kinase activity: evidence for a Ca2+, calmodulin-dependent activity at the envelope and for a Ca2+, calmodulin-independent activity in the stroma of pea chloroplasts eng 1984 author Simon Patrice 26360 author Bonzon Marc 24680 author Greppin Hubert 24907 author Marme Dieter 25425 332-338 10.1016/0014-5793(84)80152-0 0014-5793 http://doi.wiley.com/10.1016/0014-5793%2884%2980152-0 Chloroplasts were prepared from pea seedlings and tested for NAD kinase activity. More than half of a Ca²⁺, calmodulin-dependent activity and most of a Ca²⁺, calmodulin-independent activity of the homogenate were associated with chloroplasts. The Ca²⁺, calmodulin-dependent activity could be detected by adding Ca²⁺ and calmodulin to the incubation medium containing intact chloroplasts. This activity could not be separated from the chloroplasts by successive washes or by phase partition in aqueous two-polymer phase systems. After chloroplasts fractionation, the Ca²⁺, calmodulin-dependent NAD kinase activity was localized at the envelope, and the Ca²⁺, calmodulin-independent activity was recovered from the stroma. In view of these results and of a previous report [Simon (1982) Plant Cell Rep. 1, 119–122] the occurrence and presumed role of calmodulin in the chloroplast are discussed. NAD kinase Calmodulin Chloroplast envelope Pea chloroplast Dewey 580 45 Département des sciences végétales 163 false FEBS Letters 167 2 d0789c98-d4b3-4593-91c7-abf8b45148c8 Article (Published version) Restricted 1-s2.0-0014579384801520-main.pdf 645737 application/pdf restrictedAccess Faculté des sciences / Section de biologie / Département des sciences végétales

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Subchloroplastic localization of NAD kinase activity: evidence for a Ca²⁺, calmodulin-dependent activity at the envelope and for a Ca²⁺, calmodulin-independent activity in the stroma of pea chloroplasts	FEBS Letters			1984	289	0

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