Secretory organelle biogenesis: Trichocyst formation in a ciliated protozoan

Article Article scientifique Article Secretory organelle biogenesis: Trichocyst formation in a ciliated protozoan eng 1993 author Peck Robert 25553 author Swiderski Barbara author Tourmel Anne-Marie 24739 277-287 10.1016/S0248-4900(05)80199-6 0248-4900 By classical electron microscopy and immunoelectron microscopy, the biogenesis of trichocyst secretory granules has been followed in the ciliated protozoan Pseudomicrothorax dubius. The very early pre-trichocysts form by fusion of bristle-coated, electron-dense vesicles (dense vesicles) with electron-translucent vesicles (clear vesicles), both of which originate in a well-developed trans-Golgi network (TGN). The pre-trichocyst grows by further fusion with dense and clear vesicles as well as with other pre-trichocysts until it reaches its maximum diameter of about 2 μm. Dense and clear vesicle formation from the TGN has been followed, and the fusion sequence of dense vesicles with the pre-trichocyst has been documented. The contents of the dense vesicles are the precursors of the trichocyst tip, which is composed of four arm-like rods, whereas the shaft precursors are supplied by the clear vesicles. The first evidence of trichocyst shaft formation is the appearance of a paracrystalline, dense core condensation center in the pre-trichocyst. Following shaft formation, the trichocyst tip forms by fusion and condensation of the dense arm precursors along each of the four sides of the shaft. Docking of the fully formed trichocyst in the cell cortex is described. Pre-trichocyst biogenesis in cells grown with and without Se is compared. Trans-Golgi network Golgi system Coated vesicles Trichocysts Secretion Pseudomicrothorax dubius Ciliate Protozoan Dewey 615 31 Section des sciences pharmaceutiques 17 false Biology of the cell 77 3 65e17427-a0d9-47da-9895-dfc772dc3a85 Article (Published version) Restricted R.K.Peck.pdf 3152003 application/pdf restrictedAccess Faculté des sciences / Section des sciences pharmaceutiques

Title	Published in	Year	Views	Downloads

Secretory organelle biogenesis: Trichocyst formation in a ciliated protozoan	Biology of the cell	1993	552	0
Involvement of the trans-golgi network, coated vesicles, vesicle fusion and secretory product condensation in the biogenesis of Pseudomicrothorax trichocysts	Advances in Cellular and Molecular Biology of Membranes	1993	394	0

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Tourmel, Anne-Marie