Broad-host-range Rhizobium species strain NGR234 secretes a family of carbamoylated, and fucosylated, nodulation signals that are O-acetylated or sulphated

Article Article scientifique Article Broad-host-range Rhizobium species strain NGR234 secretes a family of carbamoylated, and fucosylated, nodulation signals that are O-acetylated or sulphated eng 1992 author Price Neil Philip John 24539 author Relic Biserka 24349 author Talmont F. author Lewin Astrid 37756 author Promé D. author Pueppke Steven G. author Maillet F. author Dénarié J. author Promé J.-C. author Broughton William John 22724 3575-3584 10.1111/j.1365-2958.1992.tb01793.x 0950-382X http://doi.wiley.com/10.1111/j.1365-2958.1992.tb01793.x Rhizobium species strain NGR234 is the most promiscuous known rhizobium. In addition to the non‐legume Parasponia andersonii, it nodulates at least 70 genera of legumes. Here we show that the nodulation genes of this bacterium determine the production of a large family of Nod‐factors which are N‐acylated chitin pentamers carrying a variety of substituents. The terminal non‐reducing glucosamine is N‐acylated with vaccenic or palmitic acids, is N‐methylated, and carries varying numbers of carbamoyl groups. The reducing N‐acetyl‐glucosamine residue is substituted on position 6 with 2‐O‐methyl‐L‐fucose which may be acetylated or sulphated or non‐substituted. All three internal residues are N‐acetylated. At pico‐ to nanomolar concentrations, these signal molecules exhibit biological activities on the tropical legumes Macroptilium and Vigna (Phaseoleae), as well as on both the temperate genera Medicago (Trifoliae) and Vicia (Viciae). These data strongly suggest that the uniquely broad host range of NGR234 is mediated by the synthesis of a family of varied sulphated and non‐sulphated lipo‐oligosaccharide signals. Dewey 580 45 Département des sciences végétales 163 false Molecular Microbiology 6 23 577d5a38-4a47-4f50-a777-4df727a3fe8f Article (Published version) Restricted Price_MM_1992063575.pdf 7785376 application/pdf restrictedAccess Faculté des sciences / Section de biologie / Département des sciences végétales

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Broad-host-range Rhizobium species strain NGR234 secretes a family of carbamoylated, and fucosylated, nodulation signals that are O-acetylated or sulphated	Molecular Microbiology			1992	341	0

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Price, Neil Philip John