Human Neural Organoids for Studying Brain Cancer and Neurodegenerative Diseases

Article Article scientifique Article vidéo Human Neural Organoids for Studying Brain Cancer and Neurodegenerative Diseases eng 2019-06-28 2019-06-28 author Cosset Erika 758924 author Locatelli Manon 870687 author Marteyn Antoine 862756 author Lescuyer Pierre 0000-0001-6986-8738 562350 author Dall'Antonia Florence 24512 author Mor Flavio Maurizio author Preynat-Seauve Olivier 561776 author Stoppini Luc author Tieng Caulet Tep Vannary 706263 e59682 10.3791/59682 31305527 1940-087X https://www.jove.com/t/59682/human-neural-organoids-for-studying-brain-cancer-neurodegenerative The lack of relevant in vitro neural models is an important obstacle on medical progress for neuropathologies. Establishment of relevant cellular models is crucial both to better understand the pathological mechanisms of these diseases and identify new therapeutic targets and strategies. To be pertinent, an in vitro model must reproduce the pathological features of a human disease. However, in the context of neurodegenerative disease, a relevant in vitro model should provide neural cell replacement as a valuable therapeutic opportunity. Such a model would not only allow screening of therapeutic molecules but also can be used to optimize neural protocol differentiation [for example, in the context of transplantation in Parkinson's disease (PD)]. This study describes two in vitro protocols of 1) human glioblastoma development within a human neural organoids (NO) and 2) neuron dopaminergic (DA) differentiation generating a three-dimensional (3D) organoid. For this purpose, a well-standardized protocol was established that allows the production of size-calibrated neurospheres derived from human embryonic stem cell (hESC) differentiation. The first model can be used to reveal molecular and cellular events occurring during in glioblastoma development within the neural organoid, while the DA organoid not only represents a suitable source of DA neurons for cell therapy in Parkinson's disease but also can be used for drug testing. Dewey 616 Dewey 616.07 137 Département de pathologie et immunologie 21F 56 Département de médecine 221 false 211 Auto-immunité, inflammation et athérosclérose 270 950 Génomique et métagénomique des produits sanguins labiles 956 27 Immunothérapie des cancers 42 38 Radicaux libres et cellules souches embryonnaires 60 https://www.jove.com/video/59682 Journal of visualized experiments 148 542d072c-2ef5-4952-afbc-648c8d0983a7 Article (Published version) Public Article-scientifique-from-unpaywall.pdf 1690516 application/pdf CC BY-NC-ND-3.0 d585ed5b-001d-43f4-bce8-1145f452f572 Appendix Public document(1).pdf 69765 application/pdf Materials list openAccess Faculté de médecine / Section de médecine fondamentale / Département de pathologie et immunologie Faculté de médecine / Section de médecine clinique / Département de médecine

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Human Neural Organoids for Studying Brain Cancer and Neurodegenerative Diseases	Journal of visualized experiments			2019	304	263

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