Inner Mitochondrial Membranes Bound to Concanavalin A-Sepharose Display Succinate Dehydrogenase, ATPase, and Cytochrome Oxidase Activity

Article Article scientifique Article Inner Mitochondrial Membranes Bound to Concanavalin A-Sepharose Display Succinate Dehydrogenase, ATPase, and Cytochrome Oxidase Activity eng 1992 author Strasser Reto 25398 author Millan De Ruiz Lourdes 23949 author Darszon-Israel Alberto 22841 1080-1085 10.1002/bit.260391103 0006-3592 http://doi.wiley.com/10.1002/bit.260391103 A fraction (15–20% of the total protein) of a preparation of bovine submitochondrial particles (SMPs) binds to concanavalin A–sepharose. The bound membranes displayed succinate dehydrogenase, cytochrome oxidase, and ATPase activity, which, as in SMPs, were inhibited by malonate, cyanide, and oligomycin, respectively. These results indicate that the bound membranes are inner mitochondrial membranes and that they contain a glycoprotein which was recognized by concanavalin A. It was possible to repeatedly perform the three enzyme assays, one after the other, in the same gel with the bound membranes. Long‐term stability tests (22 days) showed that cytochrome oxidase was much more stable in the membranes bound to the gel than in SMPs, while the ATPase activity decayed at a similar rate in the two conditions. Thus, inner mitochondrial membranes bound to ConA–Sepharose appear to be a potentially interesting model for the study of immobilized multienzymatic complexes. Immobilization Mitochondrial membranes Cytochrome oxidase Concanavalin A–Sepharose ATPase Dewey 580 45 Département des sciences végétales 163 false Biotechnology and Bioengineering 39 11 736efdd3-95ad-41e6-a693-9b7ad71222a5 Article (Published version) Restricted Strasser_BB_1992391080.pdf 483153 application/pdf restrictedAccess Faculté des sciences / Section de biologie / Département des sciences végétales

Title	Published in	Access level	OA Policy	Year	Views	Downloads

Inner Mitochondrial Membranes Bound to Concanavalin A-Sepharose Display Succinate Dehydrogenase, ATPase, and Cytochrome Oxidase Activity	Biotechnology and Bioengineering			1992	327	0

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