Real-time recording of circadian liver gene expression in freely moving mice reveals the phase-setting behavior of hepatocyte clocks

Article Article scientifique Article Real-time recording of circadian liver gene expression in freely moving mice reveals the phase-setting behavior of hepatocyte clocks eng 2013 author Saini Camille 510657 author Liani André 134898 author Curie Thomas author Gos Pascal 27819 author Kreppel Florian author Emmenegger Yann author Bonacina Luigi 0000-0003-0476-4473 632447 author Wolf Jean-Pierre 0000-0003-3729-958X 631266 author Poget Yves Alain 23618 author Franken Paulus 113434 author Schibler Ulrich 22687 1526-1536 10.1101/gad.221374.113 0890-9369 The mammalian circadian timing system consists of a master pacemaker in the suprachiasmatic nucleus (SCN) in the hypothalamus, which is thought to set the phase of slave oscillators in virtually all body cells. However, due to the lack of appropriate in vivo recording technologies, it has been difficult to study how the SCN synchronizes oscillators in peripheral tissues. Here we describe the real-time recording of bioluminescence emitted by hepatocytes expressing circadian luciferase reporter genes in freely moving mice. The technology employs a device dubbed RT-Biolumicorder, which consists of a cylindrical cage with reflecting conical walls that channel photons toward a photomultiplier tube. The monitoring of circadian liver gene expression revealed that hepatocyte oscillators of SCN-lesioned mice synchronized more rapidly to feeding cycles than hepatocyte clocks of intact mice. Hence, the SCN uses signaling pathways that counteract those of feeding rhythms when their phase is in conflict with its own phase. Circadian gene expression Real-time bioluminescence monitoring Liver oscillators Resetting cues SCN lesion Dewey 500.2 Dewey 570 35 Groupe de physique appliquée 135 43 Département de biologie moléculaire 161 false Genes & development 27 13 636017e5-6bae-4112-ade0-052fbedcef8f Article (Published version) Public Wolf7_2013.pdf 1367185 application/pdf openAccess Faculté des sciences / Section de physique / Groupe de physique appliquée Faculté des sciences / Section de biologie / Département de biologie moléculaire

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Real-time recording of circadian liver gene expression in freely moving mice reveals the phase-setting behavior of hepatocyte clocks	Genes & development			2013	833	1,148

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Poget, Yves Alain