Association of genes coding for the alpha-4, alpha-5, beta-2 and beta-3 subunits of nicotinic receptors with cigarette smoking and nicotine dependence

Article Article scientifique Article Association of genes coding for the alpha-4, alpha-5, beta-2 and beta-3 subunits of nicotinic receptors with cigarette smoking and nicotine dependence eng 2009 author Etter Jean-François 29008 author Hoda-Jourdan Jean-Charles 626137 author Perroud Nader author Munafò Marcus author Buresi Catherine author Duret Claudette 23476 author Neidhart Elisabeth author Malafosse Alain 111681 author Bertrand Daniel 24727 772-775 10.1016/j.addbeh.2009.05.010 19482438 0306-4603 We assessed whether smoking behavior was associated with nine polymorphisms in genes coding for the nicotinic receptor subunits alpha-4 (rs1044394, rs1044396, rs2236196 and rs2273504), alpha-5 (rs16969968), beta-2 (rs2072661 and rs4845378) and beta-3 (rs4953 and rs6474413).We conducted an Internet survey and collected saliva by mail for DNA and cotinine analyses, in Switzerland in 2003. We conducted DNA analyses for 277 participants and cotinine analyses for 141 current daily smokers. Cotinine levels were higher in carriers of the CC genotype of CHRNA4 rs1044396 (371 ng/ml) than in those with the CT or TT genotypes (275 ng/ml, p=0.049), a difference of 0.53 standard deviation units. However, this difference was not robust to correction for multiple testing using Bonferroni adjustment. These 9 polymorphisms were not otherwise associated with smoking behavior and nicotine dependence. There were possible associations between the temperament trait novelty seeking and CHRNA4 rs1044396, CHRNA5 rs16969968 and CHRNB2 rs4845378, but these associations were not robust to correction for multiple testing. We conclude that the analysis of polymorphisms in genes coding for four nicotinic acetylcholine receptor subunits (CHRNA4, CHRNA5, CHRNB2 and CHRNB3) and several smoking-related phenotypes revealed no statistically significant association. Genetics Nicotine dependence Nicotinic receptors Cigarette smoking Dewey 576.5 Dewey 613 Dewey 616.8 53 Département de neurosciences fondamentales 21A 135 Département de médecine génétique et développement 21D 66 Institut de santé globale 22A1 false 4 Génétique psychiatrique 4 90 Programme de recherche sur le tabagisme 117 Addictive behaviors 34 9 9bcb9c2b-a53a-4f0c-b451-323a0d07aca7 Article (Published version) Restricted Article.pdf 176365 application/pdf restrictedAccess Faculté de médecine / Section de médecine fondamentale / Département de neurosciences fondamentales Faculté de médecine / Section de médecine fondamentale / Département de médecine génétique et développement Faculté de médecine / Section de médecine clinique / Département de santé et médecine communautaires / Institut de santé globale

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Association of genes coding for the alpha-4, alpha-5, beta-2 and beta-3 subunits of nicotinic receptors with cigarette smoking and nicotine dependence	Addictive behaviors			2009	572	0

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Duret, Claudette