Regenerative Secretoma of Adipose-Derived Stem Cells from Ischemic Patients

Article Article scientifique Article Regenerative Secretoma of Adipose-Derived Stem Cells from Ischemic Patients eng 2019 author Uckay Ilker 134868 author Baquie Mathurin 648617 author Mosser Sébastien 911997 author Herve Marie-Priscille 745648 author Cerdan-Bruyere Pascale 24317 author Roux-Lombard Pascale 22481 author Modoux Losberger Christine 22585 author Kocjancic Curty Lasta 23059 author Ruegg Eva 816694 author Stafylakis Dimitrios 845207 author Ilmarjv Constantino author Brembilla Nicolo 703016 author Krause Karl-Heinz 23022 author Preynat-Seauve Olivier 561776 1000452 2157-7633 https://www.longdom.org/open-access/regenerative-secretoma-of-adipose-derived-stem-cells-from-ischemic-patients.pdf Patients with terminal ischemia often reveal chronic limb and foot ulcers with subsequent risk of infection and/or amputation. Adipose-derived Stem Cells (ASC) may secrete angiogenic and regenerative factors. The autologous transplantation of such cells is considered to be an attractive therapeutic strategy, but their functional properties of ASC are influenced by many biochemical and biophysical stimuli of the microenvironment. Thus, patient-derived ASC might not be functionally competent. To study ASCs in ischemic disease, we have generated ASC lines from fat tissue of twelve ischemic patients. Lines were characterized for cell surface phenotype, multipotent capacities, and production of factors involved in wound healing. We succeeded to amplify ASC lines from all twelve patients and confirmed an ASC identity by their ability to: (i) adhere and grow on a plastic surface in standard culture conditions; (ii) express an ASC expression profile; (iii) differentiate in vitro into adipocytes, osteoblasts, and chondroblasts. Full transcriptome analysis of four selected lines showed a gene expression profile compatible with healing properties including all of the functional families involved in the wound healing process: extracellular matrix proteins, cell growth factors, pro-inflammatory cytokines, angiogenic factors, and matrix remodeling proteins. Our pilot study confirms that high-quality adipose stem cells can be easily derived from ischemic patients. Their transcriptome and secretome show a regenerative profile which makes them promising candidates for autologous therapy of chronic ulcers. Chronic ulcers Chronic ischemia Adipose stem cells Neovascularization Diabetic foot infection Secretoma Proof-ofprinciple Laboratory Dewey 616 Dewey 616.07 Dewey 617 137 Département de pathologie et immunologie 21F 56 Département de médecine 221 57 Département de chirurgie 222 false 449 Etude de l'activation des monocytes/macrophages et des polynucléaires neutrophiles 779 38 Radicaux libres et cellules souches embryonnaires 60 Journal of Stem Cell Research & Therapy 9 5 1184d0f2-4503-40e8-9571-80c88c81a130 Article (Published version) Public regenerative-secretoma-of-adipose-derived-stem-cells-from-ischemic-patients.pdf 1404153 application/pdf OA OTHER openAccess Faculté de médecine / Section de médecine fondamentale / Département de pathologie et immunologie Faculté de médecine / Section de médecine clinique / Département de médecine Faculté de médecine / Section de médecine clinique / Département de chirurgie

Title	Published in	Access level	OA Policy	Year	Views	Downloads

Regenerative Secretoma of Adipose-Derived Stem Cells from Ischemic Patients	Journal of Stem Cell Research & Therapy			2019	446	294

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