GdpS contributes to Staphylococcus aureus biofilm formation by regulation of eDNA release

Article Article scientifique Article GdpS contributes to Staphylococcus aureus biofilm formation by regulation of eDNA release eng 2014 author Fischer Adrien Nicolas 639649 author Kambara Kumiko 135636 author Meyer H author Stenz Ludwig 0000-0002-2339-9451 634753 author Bonetti E-J author Girard Myriam 666631 author Lalk M author Francois Patrice 0000-0002-0540-750X 28911 author Schrenzel Jacques 29184 284-299 10.1016/j.ijmm.2013.10.010 24275081 1438-4221 In Staphylococcus aureus, the role of the GGDEF domain-containing protein GdpS remains poorly understood. Previous studies reported that gdpS mutant strains had decreased biofilm formation due to changes in icaADBC expression that were independent of cyclic-di-GMP levels. We deleted gdpS in three unrelated S. aureus isolates, and analyzed the resultant mutants for alterations in biofilm formation, metabolism and transcription. Dynamic imaging during biofilm development showed that GdpS inhibited early biofilm formation in only two out of the three strains examined, without affecting bacterial survival. However, quantification of biofilm formation using crystal violet staining revealed that inactivation of gdpS affected biofilm formation in all three studied strains. Extraction of metabolites from S. aureus cells confirmed the absence of cyclic-di-GMP, suggesting that biofilm formation in this species differs from that in other Gram-positive organisms. In addition, targeted mutagenesis demonstrated that the GGDEF domain was not required for GdpS activity. Transcriptomic analysis revealed that the vast majority of GGDEF-regulated genes were involved in virulence, metabolism, cell wall biogenesis and eDNA release. Finally, expression of lrgAB or deletion of cidABC in a strain lacking gdpS confirmed the role of GdpS on regulation of eDNA production that occurred without an increase in cell autolysis, but with a late increase in holin-mediated autolysis, in the presence of high oxacillin concentrations. In summary, S. aureus GdpS contributes to cell-to-cell interactions during early biofilm formation by influencing expression of lrgAB and cidABC mediated eDNA release. We conclude that GdpS acts as a negative regulator of eDNA release. Dewey 616 54 Département de microbiologie et médecine moléculaire 21B 56 Département de médecine 221 false 338 Génomique et microbiote 604 International journal of medical microbiology 304 3-4 a7d5dfa0-513a-4937-96c0-b58bba18f4d2 Article (Published version) Restricted 1-s2.0-S1438422113001604-main.pdf 2489798 application/pdf restrictedAccess Faculté de médecine / Section de médecine fondamentale / Département de microbiologie et médecine moléculaire Faculté de médecine / Section de médecine clinique / Département de médecine

Title	Published in	Year	Views	Downloads

GdpS contributes to Staphylococcus aureus biofilm formation by regulation of eDNA release	International journal of medical microbiology	2014	664	2
Regulation and effects of the type-three secretion system of "Rhizobium" species NGR234		2008	1,194	1,073

Archive ouverte UNIGE

Kambara, Kumiko