Nup-PI: the nucleopore-promoter interaction of genes in yeast

Article Article scientifique Article Nup-PI: the nucleopore-promoter interaction of genes in yeast eng 2006-02-03 author Schmid Manfred 528884 author Arib Ghislaine 520150 author Laemmli Caroline 135142 author Nishikawa Junichi 629865 author Durussel Thérèse 23466 author Laemmli Ulrich Karl 25441 379-391 10.1016/j.molcel.2005.12.012 16455493 1097-2765 Our previous work identified the inner basket of the NPC as a physical activation/protection station for force-tethered, epigenetically silenced genes. Here we show that a specific nucleopore-to-gene-promoter interaction (Nup-PI) is an early physiological event of gene activation. Nup-PI was discovered with chromatin endogenous cleavage (ChEC) experiments that mapped in vivo the genomic interaction sites of the nucleoporin Nup2p fused to microccocal nuclease (Nup2-MN). Strong Nup-PI, cleavage by Nup2-MN, is observed at the promoters of the GAL genes and at HXK1 upon activation of these genes with galactose. Nup-PI at the GAL locus requires Gal4p and the UASg and TATA box elements but not SAGA and active transcription. The physical, activation-dependent interaction of the GAL locus with the NPC basket was confirmed by imaging. Chromosome-wide ChEC studies indicated that Nup-PI occurs at numerous genes. The data identify the NPC basket as a new, integral participant in gene expression. Chromatin / metabolism Chromosomes, Fungal Gene Expression Regulation, Fungal Nuclear Pore / metabolism Nuclear Pore Complex Proteins / genetics Nuclear Pore Complex Proteins / metabolism Oligonucleotide Array Sequence Analysis Promoter Regions, Genetic Saccharomyces cerevisiae / genetics Saccharomyces cerevisiae / metabolism Saccharomyces cerevisiae Proteins / genetics Saccharomyces cerevisiae Proteins / metabolism Transcription, Genetic Transcriptional Activation Dewey 540 Dewey 570 39 Département de biochimie 144 43 Département de biologie moléculaire 161 false Molecular cell 21 3 d56fe501-8f0d-4845-924e-e763ce335523 Article (Published version) Public Article-scientifique-from-unpaywall.pdf 780933 application/pdf CC BY-4.0 openAccess Faculté des sciences / Section de chimie et biochimie / Département de biochimie Faculté des sciences / Section de biologie / Département de biologie moléculaire

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Nup-PI: the nucleopore-promoter interaction of genes in yeast	Molecular cell			2006	194	247

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Laemmli, Caroline