Functional expression of connexin30 and connexin31 in the polarized human airway epithelium

Article Article professionnel Article Functional expression of connexin30 and connexin31 in the polarized human airway epithelium eng 2007 author Wiszniewski Ludovic 507939 author Sanz Javier author Scerri Isabelle 519678 author Gasparotto Dabbabi Elena 134932 author Dudez Tecla 26321 author Lacroix Jean-Sylvain 528316 author Suter Susanne 22780 author Gallati Sabina author Chanson Marc 0000-0001-8104-2090 32865 382-392 10.1111/j.1432-0436.2007.00157.x 17428265 0301-4681 Gap junctions are documented in the human airway epithelium but the functional expression and molecular identity of their protein constituents (connexins, Cx) in the polarized epithelium is not known. To address this question, we documented the expression of a family of epithelial Cx (Cx26, Cx30, Cx30.3, Cx31, Cx31.1, Cx32, Cx37, Cx40, and Cx43) in primary human airway epithelial cells (AEC) grown on porous supports. Under submerged conditions, AEC formed a monolayer of airway cells whereas the air-liquid interface induced within 30-60 days AEC differentiation into a polarized epithelium for up to 6-9 months. Maturation of AEC was associated with the down-regulation of Cx26 and Cx43. The well-differentiated airway epithelium exhibited gap junctional communication between ciliated and between ciliated and basal cells. Interestingly, Cx30 was mostly present between ciliated cells whereas Cx31 was found between basal cells. These results are supportive of the establishment of signal-selective gap junctions with maturation of AEC, likely contributing to support airway epithelium function. These results lay the ground for studying the role of Cx-mediated cell-cell communication during repair following AEC injury and exploring Cx-targeted interventions to modulate the healing process. Animals Cell Communication Cell Differentiation Cells, Cultured Connexins/genetics Gap Junctions/genetics/metabolism Gene Expression Regulation/physiology Humans Mice Mice, Inbred C57BL Respiratory Mucosa/metabolism/ultrastructure Reverse Transcriptase Polymerase Chain Reaction Dewey 618 58 Département de pédiatrie, gynécologie et obstétrique 223 false 180 Mucoviscidose et jonctions GAP 229 Differentiation 75 5 1ac4a0b7-78a5-4a01-a9cc-7f5578bac5f1 Article (Published version) Restricted Wiszniewski-2007-Functional expressio.pdf 2246858 application/pdf restrictedAccess Faculté de médecine / Section de médecine clinique / Département de pédiatrie, gynécologie et obstétrique

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Functional expression of connexin30 and connexin31 in the polarized human airway epithelium	Differentiation			2007	628	3

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Gasparotto Dabbabi, Elena