Restricted access materials for fast analysis of methadone in serum with liquid chromatographymass spectrometry

Article Article scientifique Article Restricted access materials for fast analysis of methadone in serum with liquid chromatographymass spectrometry eng 2002 author Ortelli Jessica 134801 author Rudaz Serge 0000-0002-4180-5417 28978 author Souverain Sandrine author Veuthey Jean-Luc 22317 222-228 10.1002/1615-9314(20020301)25:4<222::AID-JSSC222>3.0.CO;2-# 1615-9306 Restricted access materials (RAM) which allow direct injection of biological fluids in a chromatographic system were evaluated for the direct analysis of methadone (MTD) in serum by liquid chromatography (LC) and mass spectrometry (MS). Two methods were developed for the determination of methadone and its major metabolite, EDDP, in serum samples in a concentration range of 10 to 500 ng mL± 1. The first method used the single column approach with a Pinkerton GFF II (10062.1 mm ID) analytical column. In this mode, the column is the same for the sample preparation and the analysis. The total analysis time was less than 15 min with a limit of quantification of 10 ng mL11. At this level, repeatability values were 11.8% and 15.8% for MTD and EDDP, respectively. The second method used a column-switching device. A RP-4 ADS (2562 mm ID) precolumn was coupled to a LC column and achieved a total analysis time of less than 8 minutes. At the LOQ, repeatability values were 3.2% and 7.6% for MTD and EDDP, respectively. Compared to a classical liquid-liquid extraction, RAM proved to be very convenient stationary phases. Both methods a gave short analysis time with a minimum of sample handling and allowed quantitative determination of MTD and EDDP in serum. Methadone Restricted access materials LC-MS On-line sample preparation Direct injection Column switching Dewey 615 31 Section des sciences pharmaceutiques 17 false Journal of separation science 25 8f5ae0f0-1a60-41d3-a5a7-a9656f674350 Article (Published version) Restricted fulltext.pdf 403570 application/pdf restrictedAccess Faculté des sciences / Section des sciences pharmaceutiques

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Restricted access materials for fast analysis of methadone in serum with liquid chromatographymass spectrometry	Journal of separation science			2002	650	1

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Ortelli, Jessica