Technical note: EnVision™ FLEX improves the detectability of depletions of myoglobin and troponin T in forensic cases of myocardial ischemia/infarction

Article Article scientifique Article Technical note: EnVision™ FLEX improves the detectability of depletions of myoglobin and troponin T in forensic cases of myocardial ischemia/infarction eng 2017 author Sabatasso Sara 810895 author Pomponio Catia 134740 author Fracasso Tony 731584 1643-1646 10.1007/s00414-017-1575-9 28337600 0937-9827 Immunohistochemistry is a well-established technique used in many research laboratories as well as in clinical diagnostics. The method allows to visualize the expression of proteins in biological tissues, as well as to evaluate this expression semi-quantitatively. For diagnosis, an optimal staining, based on a straightforward protocol, is crucial. In many sudden cardiac death cases, immunohistochemistry is the only tool enabling the diagnosis of myocardial ischemia/infarction, thus the diagnosis of the cause of death. Improvements in immunoreactions are actually possible thanks to optimized detection systems. The recently introduced detection system EnVision Flex™ by Dako allows to dramatically improve (in terms of intensity of the signal and practically absence of background) the visualization of antigens in formalin-fixed paraffin-embedded (FFPE) tissue sections. We tested this method for the detection of myoglobin and troponin T in human postmortem cases of myocardial infarction, as the results obtained by using the « classical » ABC (avidin-biotin complex) method have proven to be sub-optimal, thus rendering any interpretation very difficult, if not impossible. Aged Biomarkers/metabolism Female Forensic Pathology Humans Immunohistochemistry/instrumentation/methods Myocardial Infarction/pathology Myocardium/metabolism/pathology Myoglobin/metabolism Troponin T/metabolism Dewey 614.1 67 Institut de médecine légale 22A2 false 953 Groupe Fracasso Tony 959 International Journal of Legal Medicine 131 6 6e79b3a9-0c2a-4014-8352-141be220bcad Article (Published version) Private 28337600.pdf 1414464 application/pdf closedAccess Faculté de médecine / Section de médecine clinique / Département de santé et médecine communautaires / Institut de médecine légale

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Technical note: EnVision™ FLEX improves the detectability of depletions of myoglobin and troponin T in forensic cases of myocardial ischemia/infarction	International Journal of Legal Medicine			2017	443	0

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Pomponio, Catia