Dynamics of Genome Architecture in Rhizobium sp. Strain NGR234

Article Article scientifique Article Dynamics of Genome Architecture in Rhizobium sp. Strain NGR234 eng 2002 author Mavingui Aime Dieudonne Patrick 134492 author Flores Amanda Margarita 517856 author Guo Xianwu author Dávila Guillermo author Perret Xavier 23434 author Broughton William John 22724 author Palacios Rafael 171-176 10.1128/JB.184.1.171-176.2002 0021-9193 Bacterial genomes are usually partitioned in several replicons, which are dynamic structures prone to mutation and genomic rearrangements, thus contributing to genome evolution. Nevertheless, much remains to be learned about the origins and dynamics of the formation of bacterial alternative genomic states and their possible biological consequences. To address these issues, we have studied the dynamics of the genome architecture in Rhizobium sp. strain NGR234 and analyzed its biological significance. NGR234 genome consists of three replicons: the symbiotic plasmid pNGR234α (536,165 bp), the megaplasmid pNGR234b (>2,000kb), and the chromosome (>3,700 kb). Here we report that genome analyses of cell siblings showed the occurrence of large-scale DNA rearrangements consisting of cointegrations and excisions between the three replicons. As a result, four new genomic architectures have emerged. Three consisted of the cointegrates between two replicons: chromosome-pNGR234α, chromosome-pNGR234b, and pNGR234α-pNGR234b. The other consisted of a cointegrate of the three replicons (chromosome-pNGR234α-pNGR234b). Cointegration and excision of pNGR234α with either the chromosome or pNGR234b were studied and found to proceed via a Campbell-type mechanism, mediated by insertion sequence elements. We provide evidence showing that changes in the genome architecture did not alter the growth and symbiotic proficiency of Rhizobium derivatives. Dewey 580 45 Département des sciences végétales 163 false Journal of bacteriology 184 1 77612261-4a66-462f-938a-83400eb18ea9 Article (Published version) Public MAVINGUI_JB_1762002.pdf 632366 application/pdf openAccess Faculté des sciences / Section de biologie / Département des sciences végétales

Title	Published in	Access level	OA Policy	Year	Views	Downloads

Dynamics of Genome Architecture in Rhizobium sp. Strain NGR234	Journal of bacteriology			2002	103	60

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Mavingui, Aime Dieudonne Patrick