Clathrin and AP1 are required for apical sorting of glycosyl phosphatidyl inositol-anchored proteins in biosynthetic and recycling routes in Madin-Darby canine kidney cells

Article Article scientifique Article Clathrin and AP1 are required for apical sorting of glycosyl phosphatidyl inositol-anchored proteins in biosynthetic and recycling routes in Madin-Darby canine kidney cells eng 2018 author Castillon Guillaume Alain 560227 author Couleru-Burriat Patricia 134161 author Abegg Daniel 646062 author Criado Santos Nina 868630 author Watanabe Castillon Reika 560847 215-228 10.1111/tra.12548 29352747 1398-9219 Recently, studies in animal models demonstrate potential roles for clathrin and AP1 in apical protein sorting in epithelial tissue. However, the precise functions of these proteins in apical protein transport remain unclear. Here, we reveal mistargeting of endogenous glycosyl phosphatidyl inositol-anchored proteins (GPI-APs) and soluble secretory proteins in Madin-Darby canine kidney (MDCK) cells upon clathrin heavy chain or AP1 subunit knockdown (KD). Using a novel directional endocytosis and recycling assay, we found that these KD cells are not only affected for apical sorting of GPI-APs in biosynthetic pathway but also for their apical recycling and basal-to-apical transcytosis routes. The apical distribution of the t-SNARE syntaxin 3, which is known to be responsible for selective targeting of various apical-destined cargo proteins in both biosynthetic and endocytic routes, is compromised suggesting a molecular explanation for the phenotype in KD cells. Our results demonstrate the importance of biosynthetic and endocytic routes for establishment and maintenance of apical localization of GPI-APs in polarized MDCK cells. Adaptor Protein Complex 1/genetics/metabolism Animals CD59 Antigens/genetics/metabolism Clathrin/genetics/metabolism Dogs Madin Darby Canine Kidney Cells Protein Transport Synaptosomal-Associated Protein 25/genetics/metabolism Transcytosis Dewey 540 39 Département de biochimie 144 false 168 Signaux intracellulaires 210 Traffic 19 3 7ade33fd-ee6e-4606-88f5-c9c540187fcf Article (Published version) Restricted tra.12548.pdf 19867146 application/pdf restrictedAccess Faculté des sciences / Section de chimie et biochimie / Département de biochimie

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Clathrin and AP1 are required for apical sorting of glycosyl phosphatidyl inositol-anchored proteins in biosynthetic and recycling routes in Madin-Darby canine kidney cells	Traffic			2018	308	0

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Couleru-Burriat, Patricia