Uncoupling protein-3 as a molecular determinant of the action of 3,5,3'-triiodothyronine on energy metabolism

Article Article scientifique Article Uncoupling protein-3 as a molecular determinant of the action of 3,5,3'-triiodothyronine on energy metabolism eng 2009 author Flandin Pierre author Lehr Lorenz 502575 author Asensio Cédric 134037 author Giacobino Jean-Paul 24897 author Rohner-Jeanrenaud Françoise 26339 author Muzzin Patrick 23003 author Jimenez Maria 92403 246-254 10.1007/s12020-009-9217-8 19598006 1355-008X http://www.springerlink.com/content/qr75673n51n8t665/fulltext.pdf Thyroid hormones are known to stimulate thermogenesis in rodents by exerting a permissive effect on norepinephrine that affects uncoupling protein-1 (UCP1) expression in brown adipose tissue (BAT). The aim of this study was to identify new targets of the thermogenic effects of T3 in tissues other than the BAT, such as skeletal muscle. In beta(1)/beta(2)/beta(3)-adrenoceptor knockout (beta-less) mice, that are dramatically cold intolerant, a normal body temperature was maintained throughout 48 h of cold exposure by T3 administration. In these mice, BAT UCP1 protein expression was not modified either by cold exposure or by T3 administration. To test the possibility that T3 might act via muscle uncoupling protein-3 (UCP3), an UCP3 knockout (KO) model was used. This model exhibited a normal phenotype except that, upon T3 administration, stimulated oxygen consumption of the UCP3KO mice was significantly lower by 6% than that of the wild-type (WT) mice. This difference was observed only during the dark period (between 7.00 p.m. and 7.00 a.m.), i.e. when the mice are the most active at consuming food. Therefore, UCP3 might participate in the correction by T3 of the dramatic cold intolerance of the beta-less mice. These results reactivate the idea that UCP3 might play a role in the control of energy balance. Adipose Tissue, Brown/metabolism/physiology Animals Body Temperature/drug effects/genetics Cold Temperature Energy Metabolism/*drug effects/*genetics Female Ion Channels/genetics/metabolism/*physiology Mice Mice, Knockout Mitochondrial Proteins/genetics/metabolism/*physiology Oxygen Consumption/genetics/physiology Receptors, Adrenergic, beta/genetics/physiology Thermogenesis/drug effects/genetics Triiodothyronine/*pharmacology/physiology Dewey 610 Open Access - Licence nationale Springer 3 Faculté de médecine 2 false 383 Homéostasie métabolique 649 Endocrine 36 2 26eb49cb-9415-4ac3-ad67-b0811da1f741 Article (Published version) Public file1.pdf 421068 application/pdf openAccess Faculté de médecine

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Uncoupling protein-3 as a molecular determinant of the action of 3,5,3'-triiodothyronine on energy metabolism	Endocrine			2009	590	353

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