Detection of human growth hormone doping in urine: out of competition tests are necessary

Article Article scientifique Article Detection of human growth hormone doping in urine: out of competition tests are necessary eng 1996 author Saugy M. author Cardis C. author Schweizer Da Silva Catherine 133635 author Veuthey Jean-Luc 22317 author Rivier L. 201-211 10.1016/s0378-4347(96)00331-3 9001966 1570-0232 The misuse of human growth hormone (hGH) in sport is deemed to be unethical and dangerous because of various adverse effects. Thus, it has been added to the International Olympic Committee list of banned substances. Until now, the very low concentration of hGH in the urine made its measurement difficult using classical methodology. Indeed, for routine diagnosis, only plasma measurements were available. However, unlike blood samples, urine is generally provided in abundant quantities and is, at present, the only body fluid allowed to be analysed in sport doping controls. A recently developed enzyme-linked immunosorbent assay (Norditest) makes it now possible, without any extraction, to measure urinary hGH (u-hGH) in a dynamic range of 2-50 ng hGH/l. In our protocol, untreated and treated non-athlete volunteers were followed. Some of them received therapeutical doses of recombinant hGH (Norditropin) for one week either intramuscularly (three increasing doses) or subcutaneously (12 i.u. every day). The u-hGH excretion after treatment showed dramatic increases of 50-100 times the basal values and returned to almost the mean normal level after 24 h. u-hGH was also measured in samples provided by the anti-doping controls at major and minor competitions. Depending on the type of efforts made during the competition, the hGH concentration in urine was dramatically increased. Insulin-like growth factor binding proteins and beta 2-microglobulins in urine and/or in blood could be necessary for the correct investigation of any hGH doping test procedure. Adolescent Adult Doping in Sports Electrophoresis, Capillary Enzyme-Linked Immunosorbent Assay/methods Growth Hormone/administration & dosage/urine Human Growth Hormone/urine Humans Injections, Intramuscular Injections, Subcutaneous Insulin-Like Growth Factor Binding Protein 3/urine Male Middle Aged Physical Exertion Sensitivity and Specificity Substance Abuse Detection Dewey 615 31 Section des sciences pharmaceutiques 17 false Journal of chromatography. B 687 1 a93fd651-1ce1-40d4-bb2a-2cc5f83fce4e Article (Published version) Restricted fulltext.pdf 741501 application/pdf restrictedAccess Faculté des sciences / Section des sciences pharmaceutiques

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Detection of human growth hormone doping in urine: out of competition tests are necessary	Journal of chromatography. B			1996	623	0

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Schweizer Da Silva, Catherine