A novel multifunctional factor involved in trans-splicing of chloroplast introns in Chlamydomonas

Article Article scientifique Article A novel multifunctional factor involved in trans-splicing of chloroplast introns in Chlamydomonas eng 2006-01-08 author Merendino Livia 520450 author Perron Karl 45679 Département des sciences végétales (BIVEG) author Rahire Michèle 26254 author Kerautret-Bonzon Isabelle 133326 author Rochaix Jean-David 25289 Administration de la faculté des sciences (SIAD) author Goldschmidt-Clermont Michel P. 25594 262-274 10.1093/nar/gkj429 16407333 0305-1048 PMC1326246 In the chloroplast of Chlamydomonas reinhardtii , psaA mRNA is spliced in trans from three separate precursors which assemble to form two group II introns. A fourth transcript, tscA , completes the structure of the first intron. Of the fourteen nucleus-encoded factors involved in psaA splicing, only two are required for splicing of both introns. We cloned and characterized the first of these more general factors, Raa1. Consistently with its role in psaA splicing, Raa1 is imported in the chloroplast where it is found in a membrane fraction and is part of a large ribonucleoprotein complex. One mutant, raa1-L137H , is defective for splicing of both introns, but another allelic mutant, raa1-314B, still expresses the 3′ part of the Raa1 gene and is deficient only in splicing of intron 2. This observation and a deletion analysis indicate the presence of two domains in Raa1. The C-terminal domain is necessary and sufficient for processing of tscA RNA and splicing of the first intron, while the central domain is essential for splicing of the second intron. The combination of these two functional domains in Raa1 suggests that this new factor may coordinate trans-splicing of the two introns to improve the efficiency of psaA maturation. Dewey 580 Dewey 570 43 Département de biologie moléculaire 161 45 Département des sciences végétales 163 false Nucleic acids research 34 1 a29d083d-f6d3-4d6a-8f3d-81e9c8b7a12e Article (Published version) Public Merendino_NAR_200634262.pdf 2277725 application/pdf CC BY dc5a54739bbdda2e8166165d7608cd70ca8dda2283d84f310e6d06d762f64f71 openAccess Faculté des sciences / Section de biologie / Département de biologie moléculaire Faculté des sciences / Section de biologie / Département des sciences végétales

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A novel multifunctional factor involved in trans-splicing of chloroplast introns in Chlamydomonas	Nucleic acids research			2006	55	63

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Kerautret-Bonzon, Isabelle