SHREC Silences Heterochromatin via Distinct Remodeling and Deacetylation Modules

Article Article scientifique Article SHREC Silences Heterochromatin via Distinct Remodeling and Deacetylation Modules eng 2016 author Job Godwin author Brugger Christiane 771437 author Xu Tao author Lowe Brandon R. author Pfister Yvan 131571 author Qu Chunxu author Shanker Sreenath author Banos Sanz José 813294 author Partridge Janet F. author Schalch Thomas 787942 207-221 10.1016/j.molcel.2016.03.016 27105116 1097-2765 http://linkinghub.elsevier.com/retrieve/pii/S1097276516002264 Nucleosome remodeling and deacetylation (NuRD) complexes are co-transcriptional regulators implicated in differentiation, development and diseases. Methyl-CpG Binding Domain (MBD) proteins play an essential role in recruitment of NuRD complexes to their target sites in chromatin. The related SHREC complex in fission yeast drives transcriptional gene silencing in heterochromatin through cooperation with HP1 proteins. How remodeler and histone deacetylase (HDAC) cooperate within NuRD complexes remains unresolved. We determined that in SHREC the two modules occupy distant sites on the scaffold protein Clr1, and that repressive activity of SHREC can be modulated by the expression level of the HDAC-associated Clr1 domain alone. Moreover, the crystal structure of Clr2 reveals an MBD-like domain mediating recruitment of the HDAC module to heterochromatin. Thus SHREC bi-functionality is organized in two separate modules with separate recruitment mechanisms, which work together to elicit transcriptional silencing at heterochromatic loci. Heterochromatin Gene silencing Centromere X-ray crystallography Histone deacetylase Schizosaccharomyces pombe Chromatin remodeling NuRD complexes Methyl-CpG-binding proteins Dewey 570 Swiss National Science Foundation Molecular Mechanisms of Centromere Organization 139137 CH Careers 43 Département de biologie moléculaire 161 false 815 Groupe Schalch Molecular cell 62 2 2e2836b1-a8c4-4d6c-82cd-10f37590e78b Article (Accepted version) Public Job_et_al_complete_preprint.pdf 10860431 application/pdf openAccess Faculté des sciences / Section de biologie / Département de biologie moléculaire

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SHREC Silences Heterochromatin via Distinct Remodeling and Deacetylation Modules	Molecular cell			2016	585	267

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Pfister, Yvan