Interaction domains and nuclear targeting signals in subunits of the U2 small nuclear ribonucleoprotein particle-associated splicing factor SF3a

Article Article scientifique Article Interaction domains and nuclear targeting signals in subunits of the U2 small nuclear ribonucleoprotein particle-associated splicing factor SF3a eng 2011 author Huang Ching-Jung 541805 author Ferfoglia Fabio 556435 author Raleff Flore 131466 author Kraemer Angela 22808 13106-13114 10.1074/jbc.M110.201491 21349847 0021-9258 Human splicing factor SF3a is a component of the mature U2 small nuclear ribonucleoprotein particle (snRNP) and its three subunits of 60, 66, and 120 kDa are essential for splicing in vitro and in vivo. The SF3a heterotrimer forms in the cytoplasm and enters the nucleus independently of the U2 snRNP. Here, we have analyzed domains required for in vitro interactions between the SF3a subunits. Our results indicate that the SF3a66-SF3a120 interaction is mediated by a 27-amino acid region in SF3a120 C-terminal to the second suppressor-of-white-apricot and prp21/spp91 domain and amino acids 108-210 of SF3a66. Neither of these sequences contains known structural motifs, suggesting that the interaction domains are novel. Moreover, an ∼100-amino acid region, including the SURP2 domain of SF3a120 but extending into neighboring regions, is sufficient for binding to SF3a60. Analysis of determinants for nuclear import of SF3a demonstrates that SF3a120 provides the major nuclear localization signal and SF3a60 contributes to nuclear import. Active Transport, Cell Nucleus/physiology Amino Acid Motifs Cell Nucleus/genetics/metabolism Cytoplasm/genetics/metabolism Hela Cells Humans Nuclear Localization Signals/genetics/metabolism Protein Structure, Tertiary Ribonucleoprotein, U2 Small Nuclear/genetics/metabolism Dewey 570 46 Département de biologie cellulaire 165 false The Journal of biological chemistry 286 15 6c55f870-d02f-4665-9cd4-509c40bb22de Article (Published version) Restricted J. Biol. Chem.-2011-Huang-13106-14.pdf 1665016 application/pdf restrictedAccess Faculté des sciences / Section de biologie / Département de biologie cellulaire

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Interaction domains and nuclear targeting signals in subunits of the U2 small nuclear ribonucleoprotein particle-associated splicing factor SF3a	The Journal of biological chemistry			2011	592	0

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Raleff, Flore