Transcriptomic approach for assessment of the impact on microalga and macrophyte of in-situ exposure in river sites contaminated by chlor-alkali plant effluents

Article Article scientifique Article Transcriptomic approach for assessment of the impact on microalga and macrophyte of in-situ exposure in river sites contaminated by chlor-alkali plant effluents eng 2017 author Dranguet Perrine 797464 author Cosio Claudia 89306 author Le Faucheur Séverine 747213 author Beauvais-Flueck Rebecca 777564 author Freiburghaus Aline 655226 author Worms Isabelle 0000-0002-7498-0078 542783 author Petit Brice 119998 author Civic Natacha 47183 author Docquier Mylene 513819 author Slaveykova Vera 0000-0002-8361-2509 510054 86-94 10.1016/j.watres.2017.05.020 0043-1354 http://linkinghub.elsevier.com/retrieve/pii/S0043135417303792 Water quality degradation is a worldwide problem, but risk evaluation of chronic pollution in-situ is still a challenge. The present study aimed to evaluate the potential of transcriptomic analyses in representative aquatic primary producers to assess the impact of environmental pollution in-situ: the microalga Chlamydomonas reinhardtii and the macrophyte Elodea nuttallii were exposed 2 h in the Babeni Reservoir of the Olt River impacted by chlor-alkali plant effluent release resulting in increased concentrations of Hg and NaCl in receiving water. The response at the transcriptomic level was strong, resulting in up to 5485, and 8700 dysregulated genes (DG) for the microalga and for the macrophyte exposed in the most contaminated site, respectively. Transcriptomic response was congruent with the concentrations of Hg and NaCl in the water of the impacted reservoir. Genes involved in development, energy metabolism, lipid metabolism, nutrition, and RedOx homeostasis were dysregulated during in-situ exposure of both organisms. In addition, genes involved in the cell motility of C. reinhardtii and development of the cell wall of E. nuttallii were affected. DG were in line with adverse outcome pathways and transcriptomic studies reported after exposure to high concentrations of Hg and NaCl under controlled conditions in the laboratory. Transcriptomic response provided a sensitive measurement of the exposure as well as hints on the tolerance mechanisms of environmental pollution, and is thus promising as an early-warning tool to assess water quality degradation. Dewey 333.7-333.9 Dewey 550 18 Institut des sciences de l'environnement 0DB 41 Département F.-A. Forel des sciences de l'environnement et de l'eau 153 false 752 Environmental Biogeochemistry and Ecotoxicology Water research 121 f01e2939-8869-4dea-9654-948f0c176580 Article (Published version) Restricted Dranguet.pdf 757631 application/pdf restrictedAccess Centres et instituts / Institut des sciences de l'environnement Faculté des sciences / Section des sciences de la Terre et de l'Environnement / Département F.-A. Forel des sciences de l'environnement et de l'eau

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Transcriptomic approach for assessment of the impact on microalga and macrophyte of in-situ exposure in river sites contaminated by chlor-alkali plant effluents	Water research			2017	718	14

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