Microbial hitchhikers on intercontinental dust: high-throughput sequencing to catalogue microbes in small sand samples

Article Article scientifique Article Microbial hitchhikers on intercontinental dust: high-throughput sequencing to catalogue microbes in small sand samples eng 2013 author Giongo Adriana author Favet Jocelyne Louise 24703 author Lapanje Ales author Gano Kelsey A. author Kennedy Suzanne author Davis-Richardson Austin G. author Brown Christopher author Beck Andreas author Farmerie William G. author Cattaneo Arlette 26052 author Crabb David B. author Aung Yin Yin 119649 author Kort Renate author Brumsack Hans-Jürgen author Schnetger Bernhard author Broughton William John 22724 author Gorbushina Anna A. author Triplett Eric W. 71-84 10.1007/s10453-012-9264-0 0393-5965 Microbiological studies on the intercontinental transport of dust are confounded by the difficulty of obtaining sufficient material for analysis. Axenic samples of dust collected at high altitudes or historic specimens in museums are often so small and precious that the material can only be sacrificed when positive results are assured. With this in mind, we evaluated current methods and developed new ones in an attempt to catalogue all microbes present in small dust or sand samples. The methods used included classical microbiological approaches in which sand extracts were plated out on a variety of different media, polymerase chain reaction (PCR)-based amplification of 16S/18S rRNA sequences followed by construction of clone libraries, PCR amplification of 16S rRNA sequences followed by high-throughput sequencing (HtS) of the products and direct HtS of DNA extracted from the sand. A representative sand sample collected at Bahaï Wadi in the desert of the Republic of Chad was used. HtS with or without amplification showed the most promise and can be performed on ≤100 ng DNA. Since living microbes are often required, current best practices would involve geochemical and microscopic characterisation of the sample, followed by DNA isolation and direct HtS. Once the microbial content of the sample has been deciphered, growth conditions (including media) can be tailored to isolate the micro-organisms of interest. Dewey 580 Open Access - Licence nationale Springer 45 Département des sciences végétales 163 false Aerobiologia 29 1 4ae7202c-c963-4ef8-b3d0-fc571b2d8209 Article (Published version) Public Giongo2013_Article_MicrobialHitchhikersOnIntercon.pdf 501656 application/pdf openAccess Faculté des sciences / Section de biologie / Département des sciences végétales

Title	Published in	Access level	OA Policy	Year	Views	Downloads

Microbial hitchhikers on intercontinental dust: high-throughput sequencing to catalogue microbes in small sand samples	Aerobiologia			2013	443	426

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