The Ccr4-not complex and yTAF1 (yTaf(II)130p/yTaf(II)145p) show physical and functional interactions

Article Article scientifique Article The Ccr4-not complex and yTAF1 (yTaf(II)130p/yTaf(II)145p) show physical and functional interactions eng 2002 author Deluen Cécile author James Nicole author Maillet Laurent Jean Marie 512568 author Molinete Alvarez Miguel Angel 114446 author Theiler Gregory 103743 author Lemaire Marc 106697 author Paquet Nicole author Collart Martine 0000-0001-6955-1773 27009 6735-6749 10.1128/MCB.22.19.6735-6749.2002 12215531 0270-7306 The Saccharomyces cerevisiae Ccr4-Not complex is a global regulator of transcription that is thought to regulate TATA binding protein (TBP) function at certain promoters specifically. In this paper, we show interactions between the essential domain of Not1p, which interacts with Not4p and Not5p, and the N-terminal domain of yTAF1. We isolated a temperature-sensitive nonsense allele of TAF1, taf1-4, which is synthetically lethal at the permissive temperature when combined with not4 and not5 mutants and which produces high levels of a C-terminally truncated yTAF1 derivative. Overexpression of C-terminally truncated yTAF1 is toxic in not4 or not5 mutants, whereas overexpression of full-length yTAF1 suppresses not4. Furthermore, mutations in the autoinhibitory N-terminal TAND domain of yTAF1 suppress not5, and the overexpression of similar mutants does not suppress not4. We find that, like Not5p, yTAF1 acts as a repressor of stress response element-dependent transcription. Finally, we have evidence for stress-regulated occupancy of promoter DNA by Not5p and for Not5p-dependent regulation of yTAF1 association with promoter DNA. Taken together with our finding that Not1p copurifies with glutathione S-transferase-yTaf1 in large complexes, these results provide the first molecular evidence that the Ccr4-Not complex might interact with yTAF1 to regulate its association at promoters, a function that might in turn regulate the autoinhibitory N-terminal domain of yTAF1. Cell Cycle Proteins/chemistry/metabolism DNA-Binding Proteins/genetics/metabolism Fungal Proteins/genetics/metabolism Gene Expression Regulation, Fungal/physiology Macromolecular Substances Mutagenesis, Site-Directed Protein Binding/physiology Protein Structure, Tertiary/physiology Recombinant Fusion Proteins/chemistry/genetics/metabolism Repressor Proteins/genetics/metabolism Ribonucleases/metabolism Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins/genetics/metabolism TATA-Binding Protein Associated Factors Temperature Transcription Factor TFIID Transcription Factors/chemistry/genetics/metabolism Transcription Factors, TFII/chemistry/metabolism Dewey 616 Swiss National Science Foundation 31-39690.93 CH Swiss National Science Foundation 31-49808.96 CH 54 Département de microbiologie et médecine moléculaire 21B false 58 Caractérisation génétique et biochimique du complexe Ccr4-Not conservé 81 Molecular and cellular biology 22 19 6e2f1ea2-b064-4503-b428-382097d51668 Article (Published version) Restricted Deluen-2002-The Ccr4-not complex.pdf 520807 application/pdf restrictedAccess Faculté de médecine / Section de médecine fondamentale / Département de microbiologie et médecine moléculaire

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The Ccr4-not complex and yTAF1 (yTaf(II)130p/yTaf(II)145p) show physical and functional interactions	Molecular and cellular biology			2002	709	0

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Molinete Alvarez, Miguel Angel