NolL of Rhizobium sp. Strain NGR234 Is Required for O-Acetyltransferase Activity

Article Article scientifique Article NolL of Rhizobium sp. Strain NGR234 Is Required for O-Acetyltransferase Activity eng 1999-02 author Berck Sylvie Laboratoire de Biologie Moléculaire des Plantes Supérieures, Université de Genève, 1292 Chambésy, Geneva, Switzerland,1 and 111807 author Perret Xavier Laboratoire de Biologie Moléculaire des Plantes Supérieures, Université de Genève, 1292 Chambésy, Geneva, Switzerland,1 and 23434 author Vincens Quesada Dolores Laboratoire de Biologie Moléculaire des Plantes Supérieures, Université de Genève, 1292 Chambésy, Geneva, Switzerland,1 and 30112 author Promé Jean-Claude Institut de Pharmacologie et de Biologie Structurale, CNRS, 31077 Toulouse Cedex, France2 author Broughton William John Laboratoire de Biologie Moléculaire des Plantes Supérieures, Université de Genève, 1292 Chambésy, Geneva, Switzerland,1 and 22724 Administration de la faculté des sciences (SIAD) author Jabbouri Said Laboratoire de Biologie Moléculaire des Plantes Supérieures, Université de Genève, 1292 Chambésy, Geneva, Switzerland,1 and 29315 957-964 10.1128/jb.181.3.957-964.1999 0021-9193 https://journals.asm.org/doi/10.1128/JB.181.3.957-964.1999 Following (iso)flavonoid induction, nodulation genes of the symbiotic nitrogen-fixing bacterium Rhizobium sp. strain NGR234 elaborate a large family of lipooligosaccharidic Nod factors (NodNGR factors). When secreted into the rhizosphere of compatible legumes, these signal molecules initiate root hair deformation and nodule development. The nonreducing glucosamine residue of NodNGR factors are N acylated, N methylated, and mono- or biscarbamoylated, while position C-6 of the reducing extremity is fucosylated. This fucose residue is normally 2-O methylated and either sulfated or acetylated. Here we present an analysis of all acetylated NodNGR factors, which clearly shows that the acetate group may occupy position C-3 or C-4 of the fucose moiety. Disruption of the flavonoid-inducible nolL gene, which is preceded by a nod box, results in the synthesis of NodNGR factors that lack the 3- O - or 4- O -acetate groups. Interestingly, the nodulation capacity of the mutant NGRΩ nolL is not impaired, whereas introduction of the nod box:: nolL construct into the related strain Rhizobium fredii USDA257 extends the host range of this bacterium to Calopogonium caeruleum , Leucaena leucocephala , and Lotus halophilus . Nod factors produced by a USDA257(p nolL ) transconjugant were also acetylated. The nod box:: nolL construct was also introduced into ANU265 (NGR234 cured of its symbiotic plasmid), along with extra copies of the nodD1 gene. When permeabilized, these cells possessed acetyltransferase activity, although crude extracts did not. Dewey 580 45 Département des sciences végétales 163 false Journal of bacteriology 181 3 6663cad4-df41-494f-a477-e15b04995969 Article (Published version) Restricted Berck_JB_1999957.pdf 148786 application/pdf 2a9072354041563f5ffd9550fd31780939a106ef40a06340a7fc03166b0ef0b8 restrictedAccess Faculté des sciences / Section de biologie / Département des sciences végétales

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NolL of Rhizobium sp. Strain NGR234 Is Required for O-Acetyltransferase Activity	Journal of bacteriology			1999	22	0

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Berck, Sylvie