Chromatin boundaries in budding yeast: the nuclear pore connection

Article Article scientifique Article Chromatin boundaries in budding yeast: the nuclear pore connection eng 2002-05-31 author Ishii Kojiro 124710 author Arib Ghislaine 520150 author Lin Clayton 102369 author Van Houwe Griet 410315 author Laemmli Ulrich Karl 25441 551-562 10.1016/s0092-8674(02)00756-0 12062099 0092-8674 Chromatin boundary activities (BAs) were identified in Saccharomyces cerevisiae by genetic screening. Such BAs bound to sites flanking a reporter gene establish a nonsilenced domain within the silent mating-type locus HML. Interestingly, various proteins involved in nuclear-cytoplasmic traffic, such as exportins Cse1p, Mex67p, and Los1p, exhibit a robust BA. Genetic studies, immunolocalization, live imaging, and chromatin immunoprecipitation experiments show that these transport proteins block spreading of heterochromatin by physical tethering of the HML locus to the Nup2p receptor of the nuclear pore complex. Genetic deletion of NUP2 abolishes the BA of all transport proteins, while direct targeting of Nup2p to the bracketing DNA elements restores activity. The data demonstrate that physical tethering of genomic loci to the NPC can dramatically alter their epigenetic activity. Active Transport, Cell Nucleus / genetics Carrier Proteins / genetics Chromatin / genetics Chromatin / metabolism Fungal Proteins / genetics Fungal Proteins / metabolism Genes / genetics Genes, Reporter / genetics Genetic Testing Genomic Library Locus Control Region / genetics Nuclear Pore / genetics Nuclear Pore / metabolism Nuclear Pore Complex Proteins / genetics Nuclear Pore Complex Proteins / metabolism Nuclear Proteins Nucleocytoplasmic Transport Proteins Protein Binding / genetics Protein Structure, Tertiary / genetics Protein Transport / genetics Saccharomyces cerevisiae / cytology Saccharomyces cerevisiae / genetics Saccharomyces cerevisiae / metabolism Saccharomyces cerevisiae Proteins / genetics Saccharomyces cerevisiae Proteins / metabolism Dewey 540 Dewey 570 39 Département de biochimie 144 43 Département de biologie moléculaire 161 false Cell 109 5 dbae006e-a4d7-4842-8401-23ff581be702 Article (Published version) Restricted Article-scientifique-from-unpaywall.pdf 610079 application/pdf restrictedAccess Faculté des sciences / Section de chimie et biochimie / Département de biochimie Faculté des sciences / Section de biologie / Département de biologie moléculaire

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Chromatin boundaries in budding yeast: the nuclear pore connection	Cell			2002	121	0

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