Conservation of functional domains involved in RNA binding and protein-protein interactions in human and Saccharomyces cerevisiae pre-mRNA splicing factor SF1

Article Article scientifique Article Conservation of functional domains involved in RNA binding and protein-protein interactions in human and Saccharomyces cerevisiae pre-mRNA splicing factor SF1 eng 1998 author Rain J C author Rafi Zahra 102157 author Rhani Zakaria 93937 author Legrain P author Kraemer Angela 22808 551-565 10.1017/s1355838298980335 9582097 1355-8382 The modular structure of splicing factor SF1 is conserved from yeast to man and SF1 acts at early stages of spliceosome assembly in both organisms. The hnRNP K homology (KH) domain of human (h) SF1 is the major determinant for RNA binding and is essential for the activity of hSF1 in spliceosome assembly, supporting the view that binding of SF1 to RNA is essential for its function. Sequences N-terminal to the KH domain mediate the interaction between hSF1 and U2AF65, which binds to the polypyrimidine tract upstream of the 3' splice site. Moreover, yeast (y) SF1 interacts with Mud2p, the presumptive U2AF65 homologue in yeast, and the interaction domain is conserved in ySF1. The C-terminal degenerate RRMs in U2AF65 and Mud2p mediate the association with hSF1 and ySF1, respectively. Analysis of chimeric constructs of hSF1 and ySF indicates that the KH domain may serve a similar function in both systems, whereas sequences C-terminal to the KH domain are not exchangeable. Thus, these results argue for hSF1 and ySF1, as well as U2AF65 and Mud2p, being functional homologues. Amino Acid Sequence Cell Extracts Cell Nucleus/metabolism Conserved Sequence/genetics DNA-Binding Proteins Fungal Proteins/genetics/metabolism Genetic Complementation Test Hela Cells Humans Molecular Sequence Data Nuclear Proteins Point Mutation Protein Binding RNA Splicing RNA-Binding Proteins/chemistry/metabolism Recombinant Fusion Proteins Ribonucleoproteins/genetics/metabolism Saccharomyces cerevisiae/chemistry Saccharomyces cerevisiae Proteins Sequence Deletion Sequence Homology, Amino Acid Spliceosomes/metabolism Transcription Factors Dewey 570 46 Département de biologie cellulaire 165 false RNA 4 5 ec107398-22b2-4aa7-9fb9-4a49a60b729b Article (Published version) Public 1998_Rain_RNA.pdf 872451 application/pdf openAccess Faculté des sciences / Section de biologie / Département de biologie cellulaire

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Conservation of functional domains involved in RNA binding and protein-protein interactions in human and Saccharomyces cerevisiae pre-mRNA splicing factor SF1	RNA			1998	648	631

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Rafi, Zahra