Acridine-Labeled Primers as Tools for the Study of Nonenzymatic RNA Oligomerization

Article Article scientifique Article Acridine-Labeled Primers as Tools for the Study of Nonenzymatic RNA Oligomerization eng 1998 author Kurz Markus 101072 author Gobel Karin author Hartel Christian 113040 author Gobel Michael Wilhelm 101413 1156-1180 10.1002/hlca.19980810528 0018-019X http://doi.wiley.com/10.1002/hlca.19980810528 Short, dye‐labeled oligonucleotides have been used as primers in template‐controlled polymerization reactions of RNA. The synthesis of appropriate acridine derivatives and their attachments to nucleic acids is described. In the nonenzymatic oligomerization of 2‐methyl‐1H‐imidazole‐activated guanosine 5′‐monophosphate, two observations deserve special notice: (1) reaction rates are almost unchanged by variations of the Na⁺ concentration; (2) the conformational type of the primer‐template duplex (A vs. B) has considerable influence on the rates and yields of RNA oligomerization. When the incorporation of cytidine was studied in the presence of 1M Na⁺ or K⁺, the process was almost inhibited by quadruplex formation of the oligo‐dG template. However, if these cations were omitted, an efficient primer extension could be observed using template concentrations as high as 100 μM. The chances for nonenzymatic self‐replication of RNA thus might be distinctly better than previously assumed. Dewey 540 36 Département de chimie organique 141 false Helvetica Chimica Acta 81 5-8 d26c78d5-5385-4237-938e-e498a24cdea5 Article (Published version) Restricted hlca.19980810528.pdf 1519615 application/pdf restrictedAccess Faculté des sciences / Section de chimie et biochimie / Département de chimie organique

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Acridine-Labeled Primers as Tools for the Study of Nonenzymatic RNA Oligomerization	Helvetica Chimica Acta			1998	312	0

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