This study aimed to investigate the relevance of an individual's typical emotion perception and emotion regulation behavior to his or her responsiveness to humor. This was studied behaviorally by examining responses to different types of humorous stimuli in an experimental paradigm, in a sample of n = 54 participants aged between 18 to 41 years (29 women, 25 men). Individual differences in emotion perception and regulation were assessed by relevant subscales of an established self-report instrument. Higher scores on emotion perception were related to higher amusement ratings in response to the humorous stimuli. Higher scores on emotion regulation were associated with shorter response latencies for the amusement ratings, particularly when it was important to mentalize with the characters in the cartoons in order to understand the humor. The cognitive understanding of the humor was unaffected. The findings suggest that good emotion perception and emotion regulation skills may contribute to greater humor responsiveness in everyday life, which may be an adaptive trait promoting successful functioning and resilience.