Multiple short windows of Calcium-Dependent Protein Kinase 4 activity coordinate distinct cell cycle events during Plasmodium gametogenesis

Fang, Hanwei; Klages Jemelin, Natacha; Baechler, Bastien; Hillner, Evelyn; Yu, Lu; Pardo, Mercedes; Choudhary, Jyoti; Brochet, Mathieu

doi:10.7554/eLife.26524

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Title		Multiple short windows of Calcium-Dependent Protein Kinase 4 activity coordinate distinct cell cycle events during Plasmodium gametogenesis
Authors		Fang, Hanwei Klages Jemelin, Natacha Baechler, Bastien Hillner, Evelyn Yu, Lu Pardo, Mercedes Choudhary, Jyoti Brochet, Mathieu
Published in		eLife. 2017, vol. 6
Abstract		Malaria transmission relies on the production of gametes following ingestion by a mosquito. Here, we show that (Ca2+)-dependent protein kinase 4 controls three processes essential to progress from a single haploid microgametocyte to the release of eight flagellated microgametes in Plasmodium berghei. A myristoylated isoform is activated by (Ca2+) to initiate a first genome replication within twenty seconds of activation. This role is mediated by a protein of the SAPS-domain family involved in S-phase entry. At the same time, CDPK4 is required for the assembly of the subsequent mitotic spindle and to phosphorylate a microtubule-associated protein important for mitotic spindle formation. Finally, a non-myristoylated isoform is essential to complete cytokinesis by activating motility of the male flagellum. This role has been linked to phosphorylation of an uncharacterised flagellar protein. Altogether, this study reveals how a kinase integrates and transduces multiple signals to control key cell-cycle transitions during Plasmodium gametogenesis.
Keywords		Plasmodium — malaria — signalling — kinase — cell cycle — calcium — DNA replication — mitosis — gamete — flagellum — myristoylation
Identifiers		DOI: 10.7554/eLife.26524 PMID: 28481199
Full text		Article (Accepted version) (10.7 MB) - Free access
Structures		Faculté de médecine / Section de médecine fondamentale / Département de microbiologie et médecine moléculaire
Research group		Signalisation chez les parasites du paludisme (955)
Project		Swiss National Science Foundation: BSSGI0_155852
Citation (ISO format)		FANG, Hanwei et al. Multiple short windows of Calcium-Dependent Protein Kinase 4 activity coordinate distinct cell cycle events during Plasmodium gametogenesis. In: eLife, 2017, vol. 6. doi: 10.7554/eLife.26524 https://archive-ouverte.unige.ch/unige:94325