UNIGE document Scientific Article
previous document  unige:9310  next document
add to browser collection
Title

Protein kinase C activation of physiological processes in human neutrophils at vanishingly small cytosolic Ca2+ levels

Authors
Di Virgilio, F.
Pozzan, T.
Published in Nature. 1984, vol. 310, no. 5979, p. 691-693
Abstract It has long been assumed that a rise in cytosolic free Ca2+, [Ca2+]i, is a necessary and sufficient event for the stimulation of a variety of cellular processes. The development of a technique which allows monitoring of [Ca2+]i in small intact cells has led to a critical revision of this simple postulate. We have recently shown that in neutrophils, Ca2+-ionophore-induced elevations of [Ca2+]i, quantitatively similar to those caused by chemotatic peptides, are ineffective in stimulating cell responses, which suggests that an additional signal is required for receptor-mediated activation. Here we show that subthreshold concentrations of phorbol myristate acetate (PMA) and of a Ca2+ ionophore can quantitatively mimic the effect of a physiological agonist. However, PMA at higher concentrations can trigger NADPH-oxidase activity, exocytosis and protein phosphorylation, even when [Ca2+]i is lowered 10-20 times below the normal resting level. These results strongly suggest that activation of protein kinase C is sufficient, by itself, to induce NADPH-oxidase activation and exocytosis of secondary granules in neutrophils.
Keywords Calcium/ blood/pharmacologyCytosol/enzymologyHumansKineticsN-Formylmethionine Leucyl-Phenylalanine/pharmacologyNeutrophils/drug effects/ metabolismOxygen Consumption/drug effectsProtein Kinase CProtein Kinases/ bloodTetradecanoylphorbol Acetate/pharmacology
Identifiers
PMID: 6236373
Full text
Structures
Citation
(ISO format)
DI VIRGILIO, F., LEW, Daniel Pablo, POZZAN, T. Protein kinase C activation of physiological processes in human neutrophils at vanishingly small cytosolic Ca2+ levels. In: Nature, 1984, vol. 310, n° 5979, p. 691-693. https://archive-ouverte.unige.ch/unige:9310

181 hits

0 download

Update

Deposited on : 2010-07-12

Export document
Format :
Citation style :