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Epithelial autophagy and longevity of Hydra oligactis, a new model for aging research

Defense Thèse de doctorat : Univ. Genève, 2017 - Sc. 5047 - 2017/02/03
Abstract Hydra, a freshwater cnidarian possesses high regenerative potential and negligible aging. However, the Cold Sensitive H. oligactis strain (Ho_CS) induces aging upon the loss of interstitial stem cell lineage. Aging in Ho_CS is characterized by the loss of budding, regeneration and degeneration followed by death of animals within three months. This result is surprising as epithelial cells of most Hydra strains adapt to the loss of i-cells and sustain the animal. We measured proliferation, autophagy and protein aggregation in epithelial cells and found all three processes modified upon aging induction. Our cellular and molecular analysis provides evidence for deficient epithelial autophagy driving aging in Ho_CS. Chronic exposure to Rapamycin positively impacts the lifespan and fitness by modifying engulfment behavior, lipid metabolism and self-renewal of epithelial stem cells in autophagy independent fashion. We demonstrate that H. oligactis provides potent model system to study the relationship between autophagy, stem cells and aging.
Keywords Hydra oligactis aging and non-aging strainsInducible-agingInvertebrate model systemLoss of regenerationSelf-renewal of epithelial stem cellsAutophagy flux blockadeRapamycin-induced engulfmentLipid dropletsP62/SQSTM1ULK1 regulationLoss of interstitial stem cellsProteostasis
URN: urn:nbn:ch:unige-925147
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Research group Groupe Galliot
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TOMCZYK, Szymon. Epithelial autophagy and longevity of Hydra oligactis, a new model for aging research. Université de Genève. Thèse, 2017. doi: 10.13097/archive-ouverte/unige:92514 https://archive-ouverte.unige.ch/unige:92514

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Deposited on : 2017-03-13

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