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Scientific article
English

Binding of PTEN to specific PDZ domains contributes to PTEN protein stability and phosphorylation by microtubule-associated serine/threonine kinases

Published inThe Journal of biological chemistry, vol. 280, no. 32, p. 28936-28943
Publication date2005
Abstract

The tumor suppressor phosphatase PTEN is a key regulator of cell growth and apoptosis that interacts with PDZ domains from regulatory proteins, including MAGI-1/2/3, hDlg, and MAST205. Here we identified novel PTEN-binding PDZ domains within the MAST205-related proteins, syntrophin-associated serine/threonine kinase and MAST3, characterized the regions of PTEN involved in its interaction with distinctive PDZ domains, and analyzed the functional consequences on PTEN of PDZ domain binding. Using a panel of PTEN mutations, as well as PTEN chimeras containing distinct domains of the related protein TPTE, we found that the PTP and C2 domains of PTEN do not affect PDZ domain binding and that the C-terminal tail of PTEN (residues 350-403) provides selectivity to recognize specific PDZ domains from MAGI-2, hDlg, and MAST205. Binding of PTEN to the PDZ-2 domain from MAGI-2 increased PTEN protein stability. Furthermore, binding of PTEN to the PDZ domains from microtubule-associated serine/threonine kinases facilitated PTEN phosphorylation at its C terminus by these kinases. Our results suggest an important role for the C-terminal region of PTEN in the selective association with scaffolding and/or regulatory molecules and provide evidence that PDZ domain binding stabilizes PTEN and targets this tumor suppressor for phosphorylation by microtubule-associated serine/threonine kinases.

Keywords
  • Adaptor Proteins, Signal Transducing
  • Amino Acid Motifs
  • Animals
  • COS Cells
  • Carrier Proteins
  • Dystrophin-Associated Proteins/ chemistry
  • Glutathione Transferase/metabolism
  • Guanylate Kinase
  • Humans
  • Immunoprecipitation
  • Membrane Proteins
  • Microtubule-Associated Proteins/metabolism
  • Microtubules/ metabolism
  • Models, Biological
  • Mutation
  • Nucleoside-Phosphate Kinase/metabolism
  • PTEN Phosphohydrolase
  • Phosphoric Monoester Hydrolases/chemistry/ metabolism
  • Phosphorylation
  • Plasmids/metabolism
  • Protein Binding
  • Protein Structure, Tertiary
  • Protein-Serine-Threonine Kinases/metabolism
  • Proteins/metabolism
  • Recombinant Fusion Proteins/chemistry
  • Saccharomyces cerevisiae/metabolism
  • Time Factors
  • Transfection
  • Tumor Suppressor Proteins/chemistry/ metabolism
  • Two-Hybrid System Techniques
Citation (ISO format)
VALIENTE, Miguel et al. Binding of PTEN to specific PDZ domains contributes to PTEN protein stability and phosphorylation by microtubule-associated serine/threonine kinases. In: The Journal of biological chemistry, 2005, vol. 280, n° 32, p. 28936–28943. doi: 10.1074/jbc.M504761200
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Identifiers
ISSN of the journal0021-9258
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