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Scientific article
English

Sites of synthesis of urokinase and tissue-type plasminogen activators in the murine kidney

Published inThe Journal of clinical investigation, vol. 87, no. 3, p. 962-970
Publication date1991
Abstract

Kidneys have long been recognized as a major source of plasminogen activators (PAs). However, neither the sites of synthesis of the enzymes nor their role in renal function have been elucidated. By the combined use of zymographies on tissue sections and in situ hybridizations, we have explored the cellular distribution of urokinase-type (u-PA) and tissue-type (t-PA) plasminogen activators and of their mRNAs in developing and adult mouse kidneys. In 17.5-d old embryos, renal tubules synthesize u-PA, while S-shaped bodies produce t-PA. In the adult kidney, u-PA is synthesized and released in urine by the epithelial cells lining the straight parts of both proximal and distal tubules. In contrast, t-PA is produced by glomerular cells and by epithelial cells lining the distal part of collecting ducts. The precise segmental distribution of PAs suggests that both enzymes may be implicated in the maintenance of tubular patency, by catalyzing extracellular proteolysis to prevent or circumvent protein precipitation.

Keywords
  • Animals
  • Blotting, Northern
  • Epithelium/metabolism
  • Gestational Age
  • Kidney/embryology/ metabolism
  • Mice
  • Nucleic Acid Hybridization
  • RNA, Messenger/genetics/metabolism
  • Tissue Plasminogen Activator/ biosynthesis/genetics/urine
  • Urokinase-Type Plasminogen Activator/ biosynthesis/genetics/urine
Citation (ISO format)
SAPPINO, Pascal et al. Sites of synthesis of urokinase and tissue-type plasminogen activators in the murine kidney. In: The Journal of clinical investigation, 1991, vol. 87, n° 3, p. 962–970. doi: 10.1172/JCI115104
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ISSN of the journal0021-9738
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