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Scientific article
Open access
English

Pioneering Activity of the C-Terminal Domain of EBF1 Shapes the Chromatin Landscape for B Cell Programming

Published inImmunity, vol. 44, no. 3, p. 527-541
Publication date2016
Abstract

Lymphopoiesis requires the activation of lineage-specific genes embedded in naive, inaccessible chromatin or in primed, accessible chromatin. The mechanisms responsible for de novo gain of chromatin accessibility, known as "pioneer" function, remain poorly defined. Here, we showed that the EBF1 C-terminal domain (CTD) is required for the regulation of a specific gene set involved in B cell fate decision and differentiation, independently of activation and repression functions. Using genome-wide analysis of DNaseI hypersensitivity and DNA methylation in multipotent Ebf1(-/-) progenitors and derivative EBF1wt- or EBF1ΔC-expressing cells, we found that the CTD promoted chromatin accessibility and DNA demethylation in previously naive chromatin. The CTD allowed EBF1 to bind at inaccessible genomic regions that offer limited co-occupancy by other transcription factors, whereas the CTD was dispensable for EBF1 binding at regions that are occupied by multiple transcription factors. Thus, the CTD enables EBF1 to confer permissive lineage-specific changes in progenitor chromatin landscape.

Keywords
  • Animals
  • B-Lymphocytes/physiology
  • Cell Differentiation/genetics
  • Cell Lineage/genetics
  • Cells, Cultured
  • Chromatin/metabolism
  • DNA Methylation/genetics
  • Gene Regulatory Networks/genetics
  • Lymphoid Progenitor Cells/physiology
  • Lymphopoiesis
  • Mice
  • Mice, Knockout
  • Mice, Transgenic
  • Protein Structure, Tertiary/genetics
  • Trans-Activators/genetics/metabolism
Funding
  • Swiss National Science Foundation - Crosstalk between genetic and epigenetic events in gene expression control and DNA damage response
Citation (ISO format)
BOLLER, Sören et al. Pioneering Activity of the C-Terminal Domain of EBF1 Shapes the Chromatin Landscape for B Cell Programming. In: Immunity, 2016, vol. 44, n° 3, p. 527–541. doi: 10.1016/j.immuni.2016.02.021
Main files (1)
Article (Accepted version)
Identifiers
ISSN of the journal1074-7613
549views
162downloads

Technical informations

Creation10/28/2016 11:27:00 AM
First validation10/28/2016 11:27:00 AM
Update time03/15/2023 1:03:18 AM
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