

![]() |
Two-dimensional electrophoresis southern transfer method for detecting human genome variability using a LINE-1 sequence probe |
|
Authors | ||
Published in | Analytical biochemistry. 1995, vol. 227, no. 2, p. 319-327 | |
Abstract | A "high-resolution, two-dimensional Southern transfer" method has been developed and was used to examine the distribution of a class of interspersed repeated sequences in human genomes. This method consists of two separate restriction enzyme digestions, including an in situ digestion, and two-dimensional electrophoresis using a large-sized agarose gel. The first 163-base-pair region of the human LINE-1 full-length sequence was used to probe human genomic DNA from placental tissue samples. About 900 LINE-1 signals were resolved from each DNA sample within a 2-D plane. The bulk of the fragments were between 0.5 to 23 kilobases in length. At a minimum 15 variant signals were detected between DNA samples from male and female individuals and at a minimum 16 variant signals were detected between two different female samples. This approach can potentially be used to perform high-resolution human genome fingerprinting analyses. | |
Keywords | Base Sequence — Blotting, Southern/ methods — DNA Probes — Electrophoresis, Gel, Two-Dimensional — Female — Genetic Variation — Genome, Human — Humans — Male — Molecular Sequence Data — Repetitive Sequences, Nucleic Acid | |
Identifiers | PMID: 7573953 | |
Full text | ||
Structures | ||
Citation (ISO format) | NAKASHIMA, H. et al. Two-dimensional electrophoresis southern transfer method for detecting human genome variability using a LINE-1 sequence probe. In: Analytical biochemistry, 1995, vol. 227, n° 2, p. 319-327. doi: 10.1006/abio.1995.1287 https://archive-ouverte.unige.ch/unige:8919 |