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Scientific article
English

Trafficking/sorting and granule biogenesis in the beta-cell

Published inSeminars in cell & developmental biology, vol. 11, no. 4, p. 243-251
Publication date2000
Abstract

Proinsulin is packaged into nascent (immature, clathrin-coated) secretory granules in the trans-Golgi network (TGN) of the beta -cell along with other granular constituents including the proinsulin conversion enzymes. It is assumed that such packaging is dependent on an active sorting process, separating granular proteins from other secretory or membrane proteins, but the mechanism remains elusive. As granules mature, the clathrin coat is lost, the intragranular milieu is progressively acidified, and proinsulin is converted to insulin and C-peptide. Loss of clathrin is believed to arise by budding of clathrin-coated vesicles from maturing granules, carrying with them any inappropriate or unnecessary products and providing an additional means for refinement of granular content.

Keywords
  • Animals
  • Biological Transport, Active
  • Clathrin/metabolism
  • Golgi Apparatus/metabolism
  • Humans
  • Insulin/biosynthesis
  • Islets of Langerhans/ metabolism/ultrastructure
  • Membrane Lipids/metabolism
  • Proinsulin/metabolism
  • Secretory Vesicles/ metabolism
Citation (ISO format)
MOLINETE, M. et al. Trafficking/sorting and granule biogenesis in the beta-cell. In: Seminars in cell & developmental biology, 2000, vol. 11, n° 4, p. 243–251. doi: 10.1006/scdb.2000.0173
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ISSN of the journal1084-9521
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