Scientific article

The kidney is a major site of alpha(2)-antiplasmin production

Published inThe Journal of clinical investigation, vol. 97, no. 11, p. 2478-2484
Publication date1996

The serpin alpha2-antiplasmin (alpha2-AP) is the major circulating inhibitor of plasmin; it plays a determining role in the regulation of intravascular fibrinolysis, In addition to blood plasma, plasmin formation occurs in various organs where it is thought to fulfill a spectrum of functions not restricted to clot lysis. Alpha2-AP is synthesized by hepatocytes, but other possible sites of production have not been investigated. To explore the potential extravascular contribution of alpha2-AP in the regulation of proteolysis, we have isolated the murine alpha2-AP cDNA and determined its mRNA distribution in adult tissues. In addition to liver, kidneys are major sites of alpha2-AP mRNA accumulation in the mouse. The transcript is present in epithelial cells lining the convoluted portion of proximal tubules, and its accumulation is under androgen control. Human kidneys also contain high levels of alpha2-AP mRNA. Moderate amounts Of alpha2-AP mRNA are detected in other murine tissues such as muscle, intestine, central nervous system, and placenta. Our observations indicate that alpha2-AP can be synthesized in a number of tissues, where it could function as a distal regulator of plasmin-mediated extracellular proteolysis.

  • Amino Acid Sequence
  • Animals
  • Cattle
  • Cloning, Molecular
  • Female
  • Humans
  • Kidney/cytology/ metabolism
  • Liver/cytology/ metabolism
  • Male
  • Mice
  • Mice, Inbred Strains
  • Molecular Sequence Data
  • Orchiectomy
  • Organ Specificity
  • Pregnancy
  • RNA, Messenger/analysis/biosynthesis
  • Sequence Homology, Amino Acid
  • Tissue Plasminogen Activator/biosynthesis
  • Transcription, Genetic
  • Urokinase-Type Plasminogen Activator/biosynthesis
  • Alpha-2-Antiplasmin/ biosynthesis/chemistry
Citation (ISO format)
MENOUD, P. A. et al. The kidney is a major site of alpha(2)-antiplasmin production. In: The Journal of clinical investigation, 1996, vol. 97, n° 11, p. 2478–2484. doi: 10.1172/JCI118694
Main files (1)
ISSN of the journal0021-9738

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