UNIGE document Scientific Article
previous document  unige:87997  next document
add to browser collection
Title

Ampicillin-resistant Haemophilus influenzae isolates in Geneva: serotype, antimicrobial susceptibility, and β-lactam resistance mechanisms

Authors
Diene, S. M.
Published in European Journal of Clinical Microbiology & Infectious Diseases. 2015, vol. 34, no. 10, p. 1937-1945
Abstract The purpose of this study was to analyze the molecular mechanisms of ampicillin-resistant Haemophilus influenzae isolated in Geneva, Switzerland. We investigated the association between specific patterns of amino acid substitutions in penicillin-binding protein 3 (with or without β-lactamase production) and β-lactam susceptibility. Another main focus for this study was to compare the accuracy of disk diffusion and Etest methods to detect resistance to ampicillin and amoxicillin/clavulanic acid. The antibiotic susceptibility to β-lactam antibiotics of 124 H. influenzae isolates was determined by disk diffusion and Etest methods, and interpreted by European Committee on Antimicrobial Susceptibility Testing (EUCAST) and Clinical and Laboratory Standards Institute (CLSI) breakpoints. Alterations in PBP3 were investigated by sequencing the ftsI gene. Of the 124 clinical isolates analyzed, ampicillin resistance was found in 36% (45 out of 124). The rate of resistance to amoxicillin/clavulanic acid was 9% and 0.8%, using EUCAST and CLSI breakpoints respectively. For the 78 β-lactamase negative ampicillin-susceptible (BLNAS) isolates for which the Etest method indicated a high degree of susceptibility (MIC ≤ 1 mg/L), the disk diffusion method revealed resistance to ampicillin and amoxicillin/clavulanic acid in 33 cases (42%). Most common amino acid substitutions were Asn526Lys and Val547Ile, followed by Asp569Ser, Ala502Val, Asp350Asn, Met377Ile, Ile449Val, and Arg517His. The patterns observed were classified into six groups (IIa, IIb, IIc, IId, III-like, and miscellaneous). Continued characterization of both invasive and respiratory H. influenzae isolates is necessary in order to observe changes in the microbiology and epidemiology of this pathogen that could lead to clinical failure when treated by empirical antibiotic therapy.
Keywords AdolescentAdultAmpicillin/pharmacology/therapeutic useAmpicillin Resistance/geneticsAnti-Bacterial Agents/pharmacology/therapeutic useChildChild, PreschoolFemaleHaemophilus Infections/drug therapyHaemophilus influenzae/drug effects/geneticsHumansInfantMaleMicrobial Sensitivity TestsMiddle AgedSerogroupSwitzerlandYoung Adultbeta-Lactam Resistance/genetics
Identifiers
PMID: 26187432
Full text
Article (Published version) (304 Kb) - document accessible for UNIGE members only Limited access to UNIGE
Structures
Research group Analyse génomique et fonctionnelle du staphylocoque doré (604)
Citation
(ISO format)
CHERKAOUI, Abdessalam et al. Ampicillin-resistant Haemophilus influenzae isolates in Geneva: serotype, antimicrobial susceptibility, and β-lactam resistance mechanisms. In: European Journal of Clinical Microbiology & Infectious Diseases, 2015, vol. 34, n° 10, p. 1937-1945. https://archive-ouverte.unige.ch/unige:87997

71 hits

0 download

Update

Deposited on : 2016-10-05

Export document
Format :
Citation style :