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Scientific article
English

Facultative polypeptide translocation allows a single mRNA to encode the secreted and cytosolic forms of plasminogen activators inhibitor 2

Published inEMBO journal, vol. 8, no. 11, p. 3287-3294
Publication date1989
Abstract

Two forms of plasminogen activators inhibitor 2 (PAI-2) are synthesized by human and murine monocytes/macrophages: one accumulates in the cytosol, while the other is translocated into the endoplasmic reticulum, glycosylated and secreted. We show here that a single mRNA encodes both forms of PAI-2. Firstly, a single PIA-2 mRNA was detected by Northern blot hybridization and by RNase protection. Secondly, transfection of a PAI-2 cDNA led to the synthesis of both forms of PAI-2. Finally, in vitro translation of an mRNA transcript of the PAI-2 cDNA in the presence of microsomal membranes generated two topologically distinct forms of PAI-2. The cytosolic and secreted forms of PAI-2 do not result from the use of two translation start sites, since their synthesis initiates at the same AUG, in a sequence context that is conserved between the human and murine genes. Thus, the accumulation of one polypeptide into two topologically distinct cellular compartments can be achieved by facultative translocation.

Keywords
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Biological Transport
  • Blotting, Northern
  • Cell Fractionation
  • Cell Line
  • Cytosol/ metabolism
  • Endoplasmic Reticulum/ metabolism
  • Hot Temperature/adverse effects
  • Humans
  • Molecular Sequence Data
  • Monocytes/metabolism
  • Plasminogen Inactivators/ metabolism
  • Precipitin Tests
  • Protein Biosynthesis
  • RNA, Messenger/ genetics
  • Ribonucleases/metabolism
  • Sequence Homology, Nucleic Acid
  • Shock
  • Transfection
Citation (ISO format)
BELIN, Dominique et al. Facultative polypeptide translocation allows a single mRNA to encode the secreted and cytosolic forms of plasminogen activators inhibitor 2. In: EMBO journal, 1989, vol. 8, n° 11, p. 3287–3294.
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ISSN of the journal0261-4189
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