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Title

Facultative polypeptide translocation allows a single mRNA to encode the secreted and cytosolic forms of plasminogen activators inhibitor 2

Authors
Schleuning, W. D.
Published in EMBO Journal. 1989, vol. 8, no. 11, p. 3287-3294
Abstract Two forms of plasminogen activators inhibitor 2 (PAI-2) are synthesized by human and murine monocytes/macrophages: one accumulates in the cytosol, while the other is translocated into the endoplasmic reticulum, glycosylated and secreted. We show here that a single mRNA encodes both forms of PAI-2. Firstly, a single PIA-2 mRNA was detected by Northern blot hybridization and by RNase protection. Secondly, transfection of a PAI-2 cDNA led to the synthesis of both forms of PAI-2. Finally, in vitro translation of an mRNA transcript of the PAI-2 cDNA in the presence of microsomal membranes generated two topologically distinct forms of PAI-2. The cytosolic and secreted forms of PAI-2 do not result from the use of two translation start sites, since their synthesis initiates at the same AUG, in a sequence context that is conserved between the human and murine genes. Thus, the accumulation of one polypeptide into two topologically distinct cellular compartments can be achieved by facultative translocation.
Keywords Amino Acid SequenceAnimalsBase SequenceBiological TransportBlotting, NorthernCell FractionationCell LineCytosol/ metabolismEndoplasmic Reticulum/ metabolismHot Temperature/adverse effectsHumansMolecular Sequence DataMonocytes/metabolismPlasminogen Inactivators/ metabolismPrecipitin TestsProtein BiosynthesisRNA, Messenger/ geneticsRibonucleases/metabolismSequence Homology, Nucleic AcidShockTransfection
Identifiers
PMID: 2583099
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BELIN, Dominique et al. Facultative polypeptide translocation allows a single mRNA to encode the secreted and cytosolic forms of plasminogen activators inhibitor 2. In: EMBO Journal, 1989, vol. 8, n° 11, p. 3287-3294. https://archive-ouverte.unige.ch/unige:8636

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Deposited on : 2010-07-12

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