

Other version: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC401459/pdf/emboj00135-0117.pdf
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Facultative polypeptide translocation allows a single mRNA to encode the secreted and cytosolic forms of plasminogen activators inhibitor 2 |
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Published in | EMBO journal. 1989, vol. 8, no. 11, p. 3287-3294 | |
Abstract | Two forms of plasminogen activators inhibitor 2 (PAI-2) are synthesized by human and murine monocytes/macrophages: one accumulates in the cytosol, while the other is translocated into the endoplasmic reticulum, glycosylated and secreted. We show here that a single mRNA encodes both forms of PAI-2. Firstly, a single PIA-2 mRNA was detected by Northern blot hybridization and by RNase protection. Secondly, transfection of a PAI-2 cDNA led to the synthesis of both forms of PAI-2. Finally, in vitro translation of an mRNA transcript of the PAI-2 cDNA in the presence of microsomal membranes generated two topologically distinct forms of PAI-2. The cytosolic and secreted forms of PAI-2 do not result from the use of two translation start sites, since their synthesis initiates at the same AUG, in a sequence context that is conserved between the human and murine genes. Thus, the accumulation of one polypeptide into two topologically distinct cellular compartments can be achieved by facultative translocation. | |
Keywords | Amino Acid Sequence — Animals — Base Sequence — Biological Transport — Blotting, Northern — Cell Fractionation — Cell Line — Cytosol/ metabolism — Endoplasmic Reticulum/ metabolism — Hot Temperature/adverse effects — Humans — Molecular Sequence Data — Monocytes/metabolism — Plasminogen Inactivators/ metabolism — Precipitin Tests — Protein Biosynthesis — RNA, Messenger/ genetics — Ribonucleases/metabolism — Sequence Homology, Nucleic Acid — Shock — Transfection | |
Identifiers | PMID: 2583099 | |
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![]() ![]() Other version: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC401459/pdf/emboj00135-0117.pdf |
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Citation (ISO format) | BELIN, Dominique et al. Facultative polypeptide translocation allows a single mRNA to encode the secreted and cytosolic forms of plasminogen activators inhibitor 2. In: EMBO Journal, 1989, vol. 8, n° 11, p. 3287-3294. https://archive-ouverte.unige.ch/unige:8636 |