Facultative polypeptide translocation allows a single mRNA to encode the secreted and cytosolic forms of plasminogen activators inhibitor 2

Belin, Dominique; Wohlwend, Annelise Isabelle; Schleuning, W. D.; Kruithof, Egbert; Vassalli, Jean-Dominique

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Title		Facultative polypeptide translocation allows a single mRNA to encode the secreted and cytosolic forms of plasminogen activators inhibitor 2
Authors		Belin, Dominique Wohlwend, Annelise Isabelle Schleuning, W. D. Kruithof, Egbert Vassalli, Jean-Dominique
Published in		EMBO Journal. 1989, vol. 8, no. 11, p. 3287-3294
Abstract		Two forms of plasminogen activators inhibitor 2 (PAI-2) are synthesized by human and murine monocytes/macrophages: one accumulates in the cytosol, while the other is translocated into the endoplasmic reticulum, glycosylated and secreted. We show here that a single mRNA encodes both forms of PAI-2. Firstly, a single PIA-2 mRNA was detected by Northern blot hybridization and by RNase protection. Secondly, transfection of a PAI-2 cDNA led to the synthesis of both forms of PAI-2. Finally, in vitro translation of an mRNA transcript of the PAI-2 cDNA in the presence of microsomal membranes generated two topologically distinct forms of PAI-2. The cytosolic and secreted forms of PAI-2 do not result from the use of two translation start sites, since their synthesis initiates at the same AUG, in a sequence context that is conserved between the human and murine genes. Thus, the accumulation of one polypeptide into two topologically distinct cellular compartments can be achieved by facultative translocation.
Keywords		Amino Acid Sequence — Animals — Base Sequence — Biological Transport — Blotting, Northern — Cell Fractionation — Cell Line — Cytosol/ metabolism — Endoplasmic Reticulum/ metabolism — Hot Temperature/adverse effects — Humans — Molecular Sequence Data — Monocytes/metabolism — Plasminogen Inactivators/ metabolism — Precipitin Tests — Protein Biosynthesis — RNA, Messenger/ genetics — Ribonucleases/metabolism — Sequence Homology, Nucleic Acid — Shock — Transfection
Identifiers		PMID: 2583099
Full text		Article - Limited access to UNIGE Other version: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC401459/pdf/emboj00135-0117.pdf
Structures		Faculté de médecine / Section de médecine clinique / Département de médecine interne des spécialités
Citation (ISO format)		BELIN, Dominique et al. Facultative polypeptide translocation allows a single mRNA to encode the secreted and cytosolic forms of plasminogen activators inhibitor 2. In: EMBO Journal, 1989, vol. 8, n° 11, p. 3287-3294. https://archive-ouverte.unige.ch/unige:8636