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Separation of membrane protein complexes by native LDS-PAGE

Arnold, Janine
Eichacker, Lutz Andreas
Published in Methods in Molecular Biology. 2014, vol. 1072, p. 667-676
Abstract Gel electrophoresis has become one of the most important methods for the analysis of proteins and protein complexes in a molecular weight range of 1-10(7) kDa. The separation of membrane protein complexes remained challenging to standardize until the demonstration of Blue Native PAGE in 1991 [1] and Clear Native PAGE in 1994 [2]. We present a robust protocol for high-resolution separation of photosynthetic complexes from Arabidopsis thaliana using lithium dodecyl sulfate as anion in a modified Blue Native PAGE (LDS-PAGE). Here, non-covalently bound chlorophyll is used as a sensitive probe to characterize the assembly/biogenesis of the pigment-protein complexes essential for photosynthesis. The high fluorescence yield recorded from chlorophyll-binding protein complexes can also be used to establish the separation of native protein complexes as an electrophoretic standard.
Keywords Native LDS-PAGEThylakoid membraneChlorophyll-binding protein complexesFluorescence
PMID: 24136555
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Research groups Groupe Goldschmidt-Clermont
1Center of Organelle Research (CORE), University of Stavanger, Centre for Organelle Research, Richard Johnsensgate 4, N-4036 Stavanger, Norway
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ARNOLD, Janine et al. Separation of membrane protein complexes by native LDS-PAGE. In: Methods in Molecular Biology, 2014, vol. 1072, p. 667-676. https://archive-ouverte.unige.ch/unige:86341

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Deposited on : 2016-08-24

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