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Control of eukaryotic phosphate homeostasis by inositol polyphosphate sensor domains

Gerasimaite, Ruta
Jung, Ji-Yul
Truffault, Vincent
Pavlovic, Igor
Schmidt, Andrea
Saiardi, Adolfo
Jessen, Henning Jacob
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Published in Science. 2016, vol. 352, no. 6288, p. 986-990
Abstract Phosphorus is a macronutrient taken up by cells as inorganic phosphate (P(i)). How cells sense cellular P(i) levels is poorly characterized. Here, we report that SPX domains--which are found in eukaryotic phosphate transporters, signaling proteins, and inorganic polyphosphate polymerases--provide a basic binding surface for inositol polyphosphate signaling molecules (InsPs), the concentrations of which change in response to P(i) availability. Substitutions of critical binding surface residues impair InsP binding in vitro, inorganic polyphosphate synthesis in yeast, and P(i) transport in Arabidopsis In plants, InsPs trigger the association of SPX proteins with transcription factors to regulate P(i) starvation responses. We propose that InsPs communicate cytosolic P(i) levels to SPX domains and enable them to interact with a multitude of proteins to regulate P(i) uptake, transport, and storage in fungi, plants, and animals.
Keywords Arabidopsis/metabolismArabidopsis Proteins/chemistry/geneticsCrystallographyX-RayCytosol/metabolismHomeostasisHumansInositol/metabolismPhosphate Transport Proteins/chemistry/geneticsPhosphorus/metabolismPolyphosphates/metabolismProtein StructureSecondary/geneticsProtein StructureTertiary/geneticsSaccharomyces cerevisiae/metabolismTranscription Factors/chemistryTransferases (Other Substituted Phosphate Groups)/chemistry/genetics
PMID: 27080106
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Article (Accepted version) (8 MB) - public document Free access
Projects H2020: EUROPOLYPS
EMBO YIP Programme
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WILD, Rebekka et al. Control of eukaryotic phosphate homeostasis by inositol polyphosphate sensor domains. In: Science, 2016, vol. 352, n° 6288, p. 986-990. doi: 10.1126/science.aad9858 https://archive-ouverte.unige.ch/unige:85715

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Deposited on : 2016-08-08

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