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English

Accumulation of individual histone mRNAs during embryogenesis of the sea urchin Strongylocentrotus purpuratus

Contributors

Mauron, Alex; Kedes, Larry; Hough-Evans, Barbara R.; Davidson, Eric H.

Published in

Developmental biology, vol. 94, no. 2, p. 425-434

Publication date

1982

Abstract

We have determined that the stockpile of maternal histone mRNAs for both H2B and H3 in eggs of the sea urchin Strongylocentrotus purpuratus corresponds to 0.8–1 million molecules per egg. This value is a somewhat smaller quantity than had previously been estimated. On the other hand, the increase brought about by embryonic mRNA synthesis is 10- to 12-fold, rather than 4-fold. Further analysis of the time course of accumulation of these mRNAs suggests that histone gene expression in early development involves a complex interaction between activation of stockpiled maternal messengers, fluctuating rates of histone gene transcription, and alterations of histone mRNA stability. The maternal pool of H1 mRNA is considerably smaller (80,000 molecules). During cleavage, it partially catches up and peaks at about half the value for H2B and H3. We also examined previtellogenic oocytes for the presence of histone mRNAs by the same methods and were unable to detect any. Thus the stockpile of maternal histone mRNAs must accumulate after the previtellogenic stage of oogenesis.

Keywords

  • Animals
  • Gene expression regulation
  • Genetic code
  • Histones genetics metabolism
  • Hybridization genetic
  • Kinetics
  • RNA messenger genetics metabolism
  • Sea urchins embryology
  • Transcription genetic

UNIGE affiliation entities

Not a UNIGE publication

Citation (ISO format)

MAURON, Alex et al. Accumulation of individual histone mRNAs during embryogenesis of the sea urchin Strongylocentrotus purpuratus. In: Developmental biology, 1982, vol. 94, n° 2, p. 425–434. doi: 10.1016/0012-1606(82)90359-1

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Article (Published version)

Identifiers

Additional URL for this publication

http://www.sciencedirect.com/science/article/pii/0012160682903591

Journal ISSN

0012-1606
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Technical informations

Creation

14/06/2016 20:14:00

First validation

14/06/2016 20:14:00

Update

15/03/2023 00:33:56

Status update

15/03/2023 00:33:56

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