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Relationship of Grafted FGF-2 Overexpressing Neural Stem/Progenitor Cells with the Vasculature in the Cerebral Cortex

Publié dansCell transplantation, vol. 25, no. 7, p. 1359-1369
Date de publication2016
Résumé

Neural stem/progenitor cells (NPCs) overexpressing fibroblast growth factor 2 (FGF-2) have the distinct tendency to associate with the vasculature and establish multiple proliferative clusters in the perivascular environment after transplantation into the cerebral cortex. Strikingly, the vascular clusters of progenitor cells give rise to immature neurons after ischemic injury, raising prospect for the formation of ectopic neurogenic niches for repair. Here, we investigated the spatial relationship of perivascular clusters with the host vascular structures. FGF-2-GFP-transduced NPCs were transplanted into the intact somatosensory rat cortex. Confocal microscopic analysis revealed that grafted cells preferentially contacted with venules at sites with aquaporin-4-positive astrocytic endfeets and avoided contacts with desmin-positive pericytes. Electronmicroscopic analysis confirmed that grafted cells preferentially contact with astroglial endfeeds and only a minority of them reaches the endothelial basal membrane. These results provide new insights into the fine structure and anatomical relation of grafted FGF-2-transduced NPCs with the host vasculature.

Citation (format ISO)
TSUPYKOV, Oleg et al. Relationship of Grafted FGF-2 Overexpressing Neural Stem/Progenitor Cells with the Vasculature in the Cerebral Cortex. In: Cell transplantation, 2016, vol. 25, n° 7, p. 1359–1369. doi: 10.3727/096368916X690421
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Article (Published version)
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Identifiants
ISSN du journal0963-6897
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Informations techniques

Création30.01.2016 13:01:00
Première validation30.01.2016 13:01:00
Heure de mise à jour15.03.2023 00:26:47
Changement de statut15.03.2023 00:26:47
Dernière indexation16.01.2024 21:02:13
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