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Simultaneous Multiharmonic Imaging of Nanoparticles in Tissues for Increased Selectivity

Irondelle, Marie
Ramos Gomes, Fernanda
Bode, Julia
Passemard, Solène
Yamamoto, Yasuaki
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Published in ACS Photonics. 2015, vol. 2, no. 10, p. 1416-1422
Abstract We investigate the use of bismuth ferrite (BFO) nanoparticles for tumor tissue labeling in combination with infrared multiphoton excitation at 1250 nm. We report the efficient and simultaneous generation of second- and third-harmonic signals by the nanoparticles. On this basis, we set up a novel imaging protocol based on the co-localization of the two harmonic signals and demonstrate its benefits in terms of increased selectivity against endogenous background sources in tissue samples. Finally, we discuss the use of BFO nanoparticles as mapping reference structures for correlative light–electron microscopy.
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Research group Groupe Wolf
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ROGOV, Andrii et al. Simultaneous Multiharmonic Imaging of Nanoparticles in Tissues for Increased Selectivity. In: ACS Photonics, 2015, vol. 2, n° 10, p. 1416-1422. doi: 10.1021/acsphotonics.5b00289 https://archive-ouverte.unige.ch/unige:84338

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Deposited on : 2016-06-08

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