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Severe pneumonia due to Parachlamydia acanthamoebae following intranasal inoculation: a mice model

Pilloux, Ludovic
Sommer, Kirsten
Klos, Andreas
Stehle, Jean-Christophe
Published in Microbes and Infection. 2015, vol. 17, no. 11-12, p. 755-60
Abstract Parachlamydia acanthamoebae is an obligate intracellular bacterium naturally infecting free-living amoebae. The role of this bacterium as an agent of pneumonia is suggested by sero-epidemiological studies and molecular surveys. Furthermore, P. acanthamoebae may escape macrophages microbicidal effectors. Recently, we demonstrated that intratracheal inoculation of P. acanthamoebae induced pneumonia in 100% of infected mice. However, the intratracheal route of infection is not the natural way of infection and we therefore developed an intranasal murine model. Mice inoculated with P. acanthamoebae by intranasal inoculation lost 18% of their weight up to 8 days post-inoculation. All mice presented histological signs of pneumonia at day 2, 4, 7, and 10 post-inoculation, whereas no control mice harboured signs of pneumonia. A 5-fold increase in bacterial load was observed from day 0 to day 4 post-inoculation. Lungs of inoculated mice were positive by Parachlamydia-specific immunohistochemistry 4 days post-inoculation, and P. acanthamoebae were localized within macrophages. Thus, we demonstrated that P. acanthamoebae induce a severe pneumonia in mice. This animal model (i) further supports the role of P. acanthamoebae as an agent of pneumonia, confirming the third Koch postulate, and (ii) identified alveolar macrophages as one of the initial cells where P. acanthamoebae is localized following infection.
PMID: 26340890
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Research group Métastases du foie (657)
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PILLOUX, Ludovic et al. Severe pneumonia due to Parachlamydia acanthamoebae following intranasal inoculation: a mice model. In: Microbes and Infection, 2015, vol. 17, n° 11-12, p. 755-60. doi: 10.1016/j.micinf.2015.08.007 https://archive-ouverte.unige.ch/unige:84051

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Deposited on : 2016-05-31

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