en
Scientific article
English

DNA typing by microbead arrays and PCR-SSP: apparent false-negative or -positive hybridization or amplification signals disclose new HLA-B and -DRB1 alleles

Published inTissue antigens, vol. 71, no. 3, p. 238-241
Publication date2008
Abstract

Human leukocyte antigen (HLA) typing by polymerase chain reaction-sequence-specific oligonucleotide (PCR-SSO) hybridization on solid phase (microbead assay) or polymerase chain reaction-sequence-specific primers (PCR-SSP) requires interpretation softwares to detect all possible allele combinations. These programs propose allele calls by taking into account false-positive or false-negative signal(s). The laboratory has the option to validate typing results in the presence of strongly cross-reacting or apparent false-negative signals. Alternatively, these seemingly aberrant signals may disclose novel variants. We report here four new HLA-B (B*5620 and B*5716) and HLA-DRB1 alleles (DRB1*110107 and DRB1*1474) that were detected by apparent false-negative or -positive hybridization or amplification patterns, and ultimately resolved by sequencing. To avoid allele misassignments, a comprehensive evaluation of acquired data as documented in a quality assurance system is therefore required to confirm unambiguous typing interpretation.

Keywords
  • Alleles
  • Base Sequence
  • DNA/genetics
  • DNA Fingerprinting/methods/statistics & numerical data
  • DNA Primers/genetics
  • False Negative Reactions
  • False Positive Reactions
  • HLA-B Antigens/genetics
  • HLA-DR Antigens/genetics
  • Histocompatibility Testing/methods/statistics & numerical data
  • Humans
  • Microspheres
  • Molecular Sequence Data
  • Nucleic Acid Amplification Techniques
  • Nucleic Acid Hybridization
  • Polymerase Chain Reaction/methods
  • Sequence Homology Nucleic Acid
Citation (ISO format)
RAHAL, M. et al. DNA typing by microbead arrays and PCR-SSP: apparent false-negative or -positive hybridization or amplification signals disclose new HLA-B and -DRB1 alleles. In: Tissue antigens, 2008, vol. 71, n° 3, p. 238–241. doi: 10.1111/j.1399-0039.2007.00993.x
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Article (Accepted version)
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Identifiers
ISSN of the journal0001-2815
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