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Title

DNA typing by microbead arrays and PCR-SSP: apparent false-negative or -positive hybridization or amplification signals disclose new HLA-B and -DRB1 alleles

Authors
Rahal, M.
Kervaire, B.
Published in Tissue Antigens. 2008, vol. 71, no. 3, p. 238-41
Abstract Human leukocyte antigen (HLA) typing by polymerase chain reaction-sequence-specific oligonucleotide (PCR-SSO) hybridization on solid phase (microbead assay) or polymerase chain reaction-sequence-specific primers (PCR-SSP) requires interpretation softwares to detect all possible allele combinations. These programs propose allele calls by taking into account false-positive or false-negative signal(s). The laboratory has the option to validate typing results in the presence of strongly cross-reacting or apparent false-negative signals. Alternatively, these seemingly aberrant signals may disclose novel variants. We report here four new HLA-B (B*5620 and B*5716) and HLA-DRB1 alleles (DRB1*110107 and DRB1*1474) that were detected by apparent false-negative or -positive hybridization or amplification patterns, and ultimately resolved by sequencing. To avoid allele misassignments, a comprehensive evaluation of acquired data as documented in a quality assurance system is therefore required to confirm unambiguous typing interpretation.
Keywords AllelesBase SequenceDNA/geneticsDNA Fingerprinting/methods/statistics & numerical dataDNA Primers/geneticsFalse Negative ReactionsFalse Positive ReactionsHLA-B Antigens/geneticsHLA-DR Antigens/geneticsHistocompatibility Testing/methods/statistics & numerical dataHumansMicrospheresMolecular Sequence DataNucleic Acid Amplification TechniquesNucleic Acid HybridizationPolymerase Chain Reaction/methodsSequence Homology Nucleic Acid
Identifiers
PMID: 18179646
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Research groups Transplantation Immunologie et Osteoimmunologie (561)
Compatibilité tissulaire et transplantation clinique (562)
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(ISO format)
RAHAL, M. et al. DNA typing by microbead arrays and PCR-SSP: apparent false-negative or -positive hybridization or amplification signals disclose new HLA-B and -DRB1 alleles. In: Tissue Antigens, 2008, vol. 71, n° 3, p. 238-41. https://archive-ouverte.unige.ch/unige:833

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Deposited on : 2009-02-17

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