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Titles listDNA typing by microbead arrays and PCR-SSP: apparent false-negative or -positive hybridization or...
Titles listDNA typing by microbead arrays and PCR-SSP: apparent false-negative or -positive hybridization or...
Scientific Article
unige:833 
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DNA typing by microbead arrays and PCR-SSP: apparent false-negative or -positive hybridization or amplification signals disclose new HLA-B and -DRB1 alleles |
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| Authors | ||
| Published in | Tissue antigens. 2008, vol. 71, no. 3, p. 238-41 | |
| Abstract | Human leukocyte antigen (HLA) typing by polymerase chain reaction-sequence-specific oligonucleotide (PCR-SSO) hybridization on solid phase (microbead assay) or polymerase chain reaction-sequence-specific primers (PCR-SSP) requires interpretation softwares to detect all possible allele combinations. These programs propose allele calls by taking into account false-positive or false-negative signal(s). The laboratory has the option to validate typing results in the presence of strongly cross-reacting or apparent false-negative signals. Alternatively, these seemingly aberrant signals may disclose novel variants. We report here four new HLA-B (B*5620 and B*5716) and HLA-DRB1 alleles (DRB1*110107 and DRB1*1474) that were detected by apparent false-negative or -positive hybridization or amplification patterns, and ultimately resolved by sequencing. To avoid allele misassignments, a comprehensive evaluation of acquired data as documented in a quality assurance system is therefore required to confirm unambiguous typing interpretation. | |
| Keywords | Alleles — Base Sequence — DNA/genetics — DNA Fingerprinting/methods/statistics & numerical data — DNA Primers/genetics — False Negative Reactions — False Positive Reactions — HLA-B Antigens/genetics — HLA-DR Antigens/genetics — Histocompatibility Testing/methods/statistics & numerical data — Humans — Microspheres — Molecular Sequence Data — Nucleic Acid Amplification Techniques — Nucleic Acid Hybridization — Polymerase Chain Reaction/methods — Sequence Homology Nucleic Acid | |
| Identifiers | PMID: 18179646 | |
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| Structures | ||
| Research groups | Compatibilité tissulaire et transplantation clinique (562) Transplantation Immunologie et Osteoimmunologie (561) | |
| Citation (ISO format) | RAHAL, M. et al. DNA typing by microbead arrays and PCR-SSP: apparent false-negative or -positive hybridization or amplification signals disclose new HLA-B and -DRB1 alleles. In: Tissue antigens, 2008, vol. 71, n° 3, p. 238-41. doi: 10.1111/j.1399-0039.2007.00993.x https://archive-ouverte.unige.ch/unige:833 |
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