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Title

DNA typing by microbead arrays and PCR-SSP: apparent false-negative or -positive hybridization or amplification signals disclose new HLA-B and -DRB1 alleles
Authors
Rahal, M.
Kervaire, B.
Villard, Jean
Tiercy, Jean-Marie
Published in Tissue Antigens. 2008, vol. 71, no. 3, p. 238-41
Abstract Human leukocyte antigen (HLA) typing by polymerase chain reaction-sequence-specific oligonucleotide (PCR-SSO) hybridization on solid phase (microbead assay) or polymerase chain reaction-sequence-specific primers (PCR-SSP) requires interpretation softwares to detect all possible allele combinations. These programs propose allele calls by taking into account false-positive or false-negative signal(s). The laboratory has the option to validate typing results in the presence of strongly cross-reacting or apparent false-negative signals. Alternatively, these seemingly aberrant signals may disclose novel variants. We report here four new HLA-B (B*5620 and B*5716) and HLA-DRB1 alleles (DRB1*110107 and DRB1*1474) that were detected by apparent false-negative or -positive hybridization or amplification patterns, and ultimately resolved by sequencing. To avoid allele misassignments, a comprehensive evaluation of acquired data as documented in a quality assurance system is therefore required to confirm unambiguous typing interpretation.
Keywords Alleles — Base Sequence — DNA/genetics — DNA Fingerprinting/methods/statistics & numerical data — DNA Primers/genetics — False Negative Reactions — False Positive Reactions — HLA-B Antigens/genetics — HLA-DR Antigens/genetics — Histocompatibility Testing/methods/statistics & numerical data — Humans — Microspheres — Molecular Sequence Data — Nucleic Acid Amplification Techniques — Nucleic Acid Hybridization — Polymerase Chain Reaction/methods — Sequence Homology Nucleic Acid
Identifiers
PMID: 18179646
Full text
Article (Author postprint) (381 Kb) - document accessible for UNIGE members only Limited access to UNIGE
Other version: http://www3.interscience.wiley.com/cgi-bin/fulltext/119385674/HTMLSTART
Structures
Faculté de médecine / Section de médecine clinique / Département de médecine interne des spécialités
Research groups Transplantation Immunologie et Osteoimmunologie (561)
Compatibilité tissulaire et transplantation clinique (562)
Citation
(ISO format) 		RAHAL, M. et al. DNA typing by microbead arrays and PCR-SSP: apparent false-negative or -positive hybridization or amplification signals disclose new HLA-B and -DRB1 alleles. In: Tissue Antigens, 2008, vol. 71, n° 3, p. 238-41. https://archive-ouverte.unige.ch/unige:833

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Deposited on : 2009-02-17

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