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Title

Dentin bonding: effect of tubule orientation on hybrid-layer formation

Authors
Schüpbach, P
Lutz, F
Published in European Journal of Oral Sciences. 1997, vol. 105, no. 4, p. 344-352
Abstract In an attempt to compare the morphology of the resin-dentin interface in areas where the dentinal tubules run perpendicularly or at an angle to the cavity surface with that of areas where they run parallel to it, we studied a dentin adhesive system using transmission electron microscopy and fluorescence confocal laser scanning microscopy. The design of the study included the simulation of the normal hydrostatic pressure within the pulp and the dentinal tubules. Following acid etching of the dentinal surface with maleic acid/HEMA, the smear layer was removed, and a superficial zone was demineralized in such a way that the exposed collagenous dentin matrix retained its integrity. Confocal laser scanning microscopal investigations using primer labeled with rhodamine B showed that the penetration of the primer occurred not only vertically via surface porosities, but mainly laterally, via the dentinal tubules. The adhesive resin labeled with fluorescein completely infiltrated the demineralized layer, thereby forming a hybrid layer. The orientation of the dentinal tubules had a profound effect on the formation of the hybrid layer. In areas with perpendicular tubule orientation, the layer was 3.2 +/- 0.8 microns thick, showing solid 27.2 +/- 0.8 microns long resin tags in the dentinal tubules, and a network of tiny tags in their side-branches. In areas with parallel tubule orientation the layer was significantly thinner (1.3 +/- 0.6 microns) and resin tags were absent.
Keywords Acid Etching, DentalAdhesives/chemistryCollagen/ultrastructureDental BondingDental Cavity PreparationDental Pulp/physiologyDentin/physiology/ultrastructureDentin-Bonding Agents/chemistryFluoresceinFluorescent DyesHumansHydrostatic PressureMaleates/chemistryMethacrylates/chemistryMicroscopy, ConfocalMicroscopy, ElectronMicroscopy, FluorescencePorosityResin Cements/chemistryRhodaminesSmear LayerSurface Properties
Identifiers
PMID: 9298367
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Article (Published version) (3 MB) - public document Free access
Structures
Research group Groupe Krejci Ivo (médecine dentaire) (240)
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SCHÜPBACH, P, KREJCI, Ivo, LUTZ, F. Dentin bonding: effect of tubule orientation on hybrid-layer formation. In: European Journal of Oral Sciences, 1997, vol. 105, n° 4, p. 344-352. https://archive-ouverte.unige.ch/unige:81112

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Deposited on : 2016-03-01

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