The Páramo de Frontino is located in the northern part of the Colombian Western Cordillera. Its geographical position in the northwest of South America is strategic, because its climate is influenced by the meteorology derived principally from the Caribbean Sea and Pacific Ocean. This study presents the reconstruction of the vegetation and climate of the Páramo de Frontino during the Late Glacial. Palynology and microfluorescence-X (μXRF) are used to study the lower part of several cores at an altitude of 3460 m amsl. The age model was derived from 30 AMS 14C datations. The time interval studied spans from 16,700 till 11,500 cal yr BP. Nine palynological biozones were established based on the reconstruction of vegetation belts Variations in the vegetation belts were used as proxy for paleotemperature and local aquatic plants yielded a proxy for humidity. μXRF, especially that of the titanium, was used as another proxy for precipitations. These results have permitted the paleoenvironmental and climatic reconstruction of the Late Glacial in northwestern Colombia.