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Vitamin D represses rhinovirus replication in cystic fibrosis cells by inducing LL-37

Schögler, Aline
Muster, Ricardo J
Kieninger, Elisabeth
Casaulta, Carmen
Jung, Andreas
Moeller, Alexander
Geiser, Thomas
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Published in The European Respiratory Journal. 2016, vol. 47, no. 2, p. 520-530
Abstract Vitamin D has immunomodulatory properties in the defence against pathogens. Its insufficiency is a widespread feature of cystic fibrosis (CF) patients, which are repeatedly suffering from rhinovirus (RV)-induced pulmonary exacerbations.To investigate whether vitamin D has antiviral activity, primary bronchial epithelial cells from CF children were pre-treated with vitamin D and infected with RV16. Antiviral and anti-inflammatory activity of vitamin D was assessed. RV and LL-37 levels were measured in bronchoalveolar lavages (BALs) of CF children infected with RV.Vitamin D reduced RV16 load in a dose-dependent manner in CF cells (10(-7 )M:p<0.01). The antiviral response mediated by interferons remained unchanged by vitamin D in CF cells. Vitamin D did not exert anti-inflammatory properties in RV-infected CF cells. Vitamin D increased the expression of the antimicrobial peptide LL-37 up to 17.4-fold (p<0.05). Addition of exogenous LL-37 decreased viral replication by 4.4-fold in CF cells (p<0.05). An inverse correlation between viral load and LL-37 levels in CF BALs (r=-0.48, p<0.05) was observed.RV replication in primary CF bronchial cells was reduced by vitamin D through the induction of LL-37. Clinical studies are needed to determine the importance of an adequate control of vitamin D for prevention of virus-induced pulmonary CF exacerbations.
PMID: 26585423
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Article (Published version) (516 Kb) - public document Free access
Research group Identification des facteurs clés impliqués dans la pathogénèse des rhinovirus et entérovirus (953)
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SCHÖGLER, Aline et al. Vitamin D represses rhinovirus replication in cystic fibrosis cells by inducing LL-37. In: European Respiratory Journal, 2016, vol. 47, n° 2, p. 520-530. https://archive-ouverte.unige.ch/unige:80775

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Deposited on : 2016-02-22

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