

Other version: http://mcb.asm.org/cgi/content/full/28/5/1630?view=long&pmid=18086894
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Up-regulation of P-TEFb by the MEK1-extracellular signal-regulated kinase signaling pathway contributes to stimulated transcription elongation of immediate early genes in neuroendocrine cells |
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Published in | Molecular and cellular biology. 2008, vol. 28, no. 5, p. 1630-43 | |
Abstract | The positive elongation factor P-TEFb appears to function as a crucial C-terminal-domain (CTD) kinase for RNA polymerase II (Pol II) transcribing immediate early genes (IEGs) in neuroendocrine GH4C1 cells. Chromatin immunoprecipitation indicated that in resting cells Pol II occupied the promoter-proximal regions of the c-fos and junB genes, together with the negative elongation factors DSIF and NELF. Thyrotropin-releasing hormone (TRH)-induced recruitment of positive transcription elongation factor b (P-TEFb) abolished the pausing of Pol II and enhanced phosphorylation of CTD serine 2, resulting in transcription elongation. In addition, P-TEFb was essential for splicing and 3'-end processing of IEG transcripts. Importantly, the MEK1-extracellular signal-regulated kinase (ERK) signaling pathway activated by TRH up-regulated nuclear CDK9 and CDK9/cyclinT1 dimers (i.e., P-TEFb), facilitating the recruitment of P-TEFb to c-fos and other IEGs. Thus, in addition to established gene transcription control via promoter response elements, the MEK1-ERK signaling pathway controls transcription elongation by Pol II via the up-regulation of nuclear CDK9 integrated into P-TEFb. | |
Keywords | Animals — Cell Culture Techniques — Cells Cultured — Chromatin Immunoprecipitation — Culture Media Serum-Free — Cyclin-Dependent Kinase 9/metabolism — Extracellular Signal-Regulated MAP Kinases/metabolism — Genes Immediate-Early — Models Biological — Phosphorylation/drug effects — Pituitary Gland/cytology — Positive Transcriptional Elongation Factor B/metabolism — Precipitin Tests — Rats — Signal Transduction — Thyrotropin-Releasing Hormone/pharmacology — Time Factors — Transcription Genetic — Up-Regulation | |
Identifiers | DOI: 10.1128/MCB.01767-07 PMID: 18086894 | |
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![]() ![]() Other version: http://mcb.asm.org/cgi/content/full/28/5/1630?view=long&pmid=18086894 |
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Citation (ISO format) | FUJITA, Toshitsugu et al. Up-regulation of P-TEFb by the MEK1-extracellular signal-regulated kinase signaling pathway contributes to stimulated transcription elongation of immediate early genes in neuroendocrine cells. In: Molecular and cellular biology, 2008, vol. 28, n° 5, p. 1630-43. doi: 10.1128/MCB.01767-07 https://archive-ouverte.unige.ch/unige:794 |